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anti-Homogentisate 1,2-Dioxygenase (HGD) Antibodies

HGD encodes the enzyme homogentisate 1,2 dioxygenase. Additionally we are shipping Homogentisate 1,2-Dioxygenase Proteins (13) and Homogentisate 1,2-Dioxygenase Kits (8) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
HGD 15233 O09173
HGD 360719  
HGD 3081 Q93099
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Top anti-Homogentisate 1,2-Dioxygenase Antibodies at antibodies-online.com

Showing 10 out of 71 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-HGD Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positive Control: Hela cell lysate 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Mouse Un-conjugated ELISA, IF Immunofluorescence of monoclonal antibody to HGD on HeLa cell . [antibody concentration 10 ug/ml] Detection limit for recombinant GST tagged HGD is approximately 0.3ng/ml as a capture antibody. 100 μg Log in to see 8 to 11 Days
$527.50
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Western Blot: HGD Antibody [NBP2-33488] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.Lane 2: Human cell line RT-4.Lane 3: Human cell line U-251MG sp.Lane 4: Human plasma (IgG/HSA depleted).Lane 5: Human liver tissue Immunohistochemistry-Paraffin: HGD Antibody [NBP2-33488] - Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes. 0.1 mL Log in to see 10 to 13 Days
$494.38
Details
Mouse Rabbit Un-conjugated ICC, IHC, WB Figure. Western Blot; Sample: Lane1: Mouse Liver Tissue; Lane2: Rat Liver Tissue. 100 μg Log in to see 13 to 16 Days
$360.00
Details
Human Rabbit Un-conjugated WB 50 μg Log in to see 11 to 14 Days
$551.83
Details
Human Rabbit Un-conjugated IHC, WB Immunohistochemistry: HGD Antibody [NBP2-49039] - Staining of human liver shows cytoplasmic positivity in hepatocytes. Western Blot: HGD Antibody [NBP2-49039] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10.Lane 2: Human cell line RT-4.Lane 3: Human cell line U-251 MG.Lane 4: Human plasma.Lane 5: Human Liver tissue.Lane 6: Human Tonsil tissue 0.1 mL Log in to see 10 to 13 Days
$494.38
Details
Human Mouse Un-conjugated IP, ELISA, WB Western Blot analysis of HGD expression in transfected 293T cell line by HGD monoclonal antibody (M11), clone 1F1.Lane 1: HGD transfected lysate(50 KDa).Lane 2: Non-transfected lysate. Immunoprecipitation of HGD transfected lysate using anti-HGD monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with HGD MaxPab rabbit polyclonal antibody. 100 μg Log in to see 11 to 12 Days
$348.04
Details
Human Rabbit Un-conjugated ELISA, IHC, WB Western blot analysis of HGD using anti-HGD antibody . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each Lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HGD antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HGD at approximately 50KD. The expected band size for HGD is at 50KD. IHC analysis of HGD using anti-HGD antibody . HGD was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-HGD Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Log in to see 4 to 6 Days
$280.00
Details
Human Rabbit Un-conjugated ICC, IHC, WB Western Blot; Sample: Porcine Liver lysate; Primary Ab: 2µg/ml Rabbit Anti-Human HGD Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) 100 μg Log in to see 15 to 18 Days
$350.00
Details
Human Rabbit Un-conjugated ICC, IHC, WB Figure.DAB staining on IHC-P. Samples: Human Tissue 100 μg Log in to see 13 to 16 Days
$350.00
Details

Top referenced anti-Homogentisate 1,2-Dioxygenase Antibodies

  1. Human Polyclonal HGD Primary Antibody for WB - ABIN516420 : Pan, Zhang, Cui, Liu, McAllan, Liao, Zhang: Adaptation of phenylalanine and tyrosine catabolic pathway to hibernation in bats. in PLoS ONE 2013 (PubMed)

More Antibodies against Homogentisate 1,2-Dioxygenase Interaction Partners

Human Homogentisate 1,2-Dioxygenase (HGD) interaction partners

  1. Twelve novel HGD gene variants have been identified in 99 alkaptonuria patients affecting the bones.

  2. A novel alkaptonuria mutation, c.87 + 1G > A, shows a significant founder effect and high prevalence in a nomadic Indian population.

  3. study described two novel HGD mutations in a Chinese alkaptonuria family, the splicing mutation of IVS7 1G>C, a donor splice site of exon 7, and a missense mutation of F329C in exon 12

  4. Report mutations of the HGD gene in Jordanian alkaptonuria patients.

  5. The observed increase of HGD expression in Alkaptonuria cells is probably due to a compensatory mechanism to overcome the almost null catalytic activity of the deficient enzyme

  6. An update on molecular genetics of Alkaptonuria (AKU).(review)

  7. Familiar ochronotic arthropathy is caused by a gene mutation of the homogentisic acid 1,2-dioxygenase (HGD) gene traced three hundred years in a Hungarian family.

  8. A comprehensive mutation analysis of 93 patients enrolled in this study, as well as an extensive update of all previously published HGD mutations associated with alkaptonuria, is reported.

  9. Turkish mutation shares an homogentisate 1,2-dioxygenase haplotype with the mutation found in Finland, Slovakia and India, suggesting that R58fs is an old alkaptonuria mutation that probably originated in central Asia

  10. Four different mutations of the HGD gene were found in alkaptonuria and ochronotic arthropathy diagnoses.

  11. A single nucleotide deletion located in exon 3 resulted in a frameshift mutation in HGD gene in family with alkaptonuria.

Cow (Bovine) Homogentisate 1,2-Dioxygenase (HGD) interaction partners

  1. The effects of polymorphisims in the restriction sites for HGD-BstXI and HGD-HaeIII on meat quality and body composition of Chinese red cattle are reported.

  2. This is the first incidence where polymorphisms of a bovine HGD gene have demonstrated a correlation with meat traits in Chinese red cattle.

Homogentisate 1,2-Dioxygenase (HGD) Antigen Profile

Protein Summary

This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.

Gene names and symbols associated with HGD

  • homogentisate 1,2-dioxygenase (hgo) antibody
  • homogentisate 1,2-dioxygenase (HGD) antibody
  • homogentisate 1,2-dioxygenase (hmgA) antibody
  • homogentisate 1,2-dioxygenase (Shewana3_2481) antibody
  • homogentisate 1,2-dioxygenase (hgd) antibody
  • homogentisate 1,2-dioxygenase (BC1002_0545) antibody
  • homogentisate 1,2-dioxygenase (BC1003_0502) antibody
  • homogentisate 1,2-dioxygenase (BC1001_0489) antibody
  • Homogentisate 1,2-dioxygenase (Sinme_2995) antibody
  • Homogentisate 1,2-dioxygenase (Sphch_0441) antibody
  • homogentisate 1, 2-dioxygenase (Hgd) antibody
  • homogentisate 1,2-dioxygenase L homeolog (hgd.L) antibody
  • AKU antibody
  • CG4779 antibody
  • DDBDRAFT_0185702 antibody
  • DDBDRAFT_0191461 antibody
  • DDB_0185702 antibody
  • DDB_0191461 antibody
  • Dmel\\CG4779 antibody
  • HGO antibody
  • PSPTO3551 antibody

Protein level used designations for HGD

CG4779-PA , hgo-PA , homogentisate 1,2-dioxygenase , homogentisic acid oxidase , Homogentisate 1,2-dioxygenase , homogentisate 1,2-dioxygenase (homogentisate oxidase) , Homogentisate 1,2-dioxygenase-like , homogentisate 12-dioxygenase , alkaptonuria , homogentisate oxygenase , homogentisicase , homogentisate oxidase

GENE ID SPECIES
34552 Drosophila melanogaster
713499 Macaca mulatta
1185216 Pseudomonas syringae pv. tomato str. DC3000
3088598 Burkholderia mallei ATCC 23344
3092129 Burkholderia pseudomallei K96243
3194373 Ruegeria pomeroyi DSS-3
4476662 Shewanella sp. ANA-3
6933053 Burkholderia cenocepacia J2315
8624285 Dictyostelium discoideum AX4
9088212 Burkholderia sp. CCGE1002
9765368 Burkholderia sp. CCGE1003
10222017 Burkholderia sp. CCGE1001
10727599 Sinorhizobium meliloti AK83
10740765 Sphingobium chlorophenolicum L-1
100017306 Monodelphis domestica
100060993 Equus caballus
100135319 Xenopus (Silurana) tropicalis
100342562 Oryctolagus cuniculus
100385530 Callithrix jacchus
100528609 Ictalurus punctatus
100542509 Meleagris gallopavo
100624241 Sus scrofa
15233 Mus musculus
360719 Rattus norvegicus
259189 Danio rerio
447328 Xenopus laevis
418330 Gallus gallus
3081 Homo sapiens
507496 Bos taurus
100171592 Pongo abelii
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