anti-PRP8 Pre-MRNA Processing Factor 8 Homolog (S. Cerevisiae) (PRPF8) Antibodies

Pre-mRNA splicing occurs in 2 sequential transesterification steps. Additionally we are shipping and and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
PRPF8 287530  
PRPF8 10594 Q6P2Q9
PRPF8 192159 Q99PV0
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Top anti-PRPF8 Antibodies at antibodies-online.com

Showing 10 out of 33 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-PRPF8 Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:312500  Positive Control:  Jurkat cell lysate PRPF8 is supported by BioGPS gene expression data to be expressed in Jurkat WB Suggested Anti-PRPF8  Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500  Positive Control: Jurkat cell lysate  PRPF8 is supported by BioGPS gene expression data to be expressed in Jurkat 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated IP, WB Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody at dilution of 1:100 (x40 lens). 100 μL Log in to see 16 Days
$366.77
Details
Human Rabbit Un-conjugated IHC, WB Western blot analysis of extracts of various cell lines, using PRPF8 antibody (ABIN5973594) at 1/1000 dilution. Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody (ABIN5973594) at dilution of 1/100 (40x lens). 100 μL Log in to see 11 to 16 Days
$426.40
Details
Chicken Rabbit Un-conjugated WB 50 μg Log in to see 11 to 14 Days
$551.83
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22272] - FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-PRPF8 (Cat. No. NBP2-22272, left panel, NBP2-22273, middle panel and Cat. No. NBP2-22274, right panel) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB Western Blot: PRPF8 Antibody [NBP2-22272] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22272 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. 100 μL Log in to see 7 to 9 Days
$559.35
Details
Human Mouse Un-conjugated DB, ICC, FACS, WB   50 μg Log in to see 11 to 14 Days
$493.17
Details
Human Mouse Un-conjugated DB, ICC, FACS, WB HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-PRPF8 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG. Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-PRPF8 antibody. 50 μg Log in to see 9 to 11 Days
$285.71
Details
Human Rabbit Un-conjugated IHC, WB Western blot analysis of extracts of various cell lines, using PRPF8 antibody. Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody. 100 μL Log in to see 13 to 14 Days
$444.37
Details
Human Rabbit Un-conjugated WB PRPF8 antibody used at 1 ug/ml to detect target protein. 50 μg Log in to see 9 to 11 Days
$473.93
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), IP, WB Immunoprecipitation: PRPF8 Antibody [NBP2-22274] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PRPF8 antibody NBP2-22274 used for IP at 6 ug/mg lysate. PRPF8 was also immunoprecipitated by rabbit anti-PRPF8 antibodies NBP2-22272 and NBP2-22273 For blotting immunoprecipitated PRPF8, NBP2-22274 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds. Immunohistochemistry-Paraffin: PRPF8 Antibody [NBP2-22274] - FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-PRPF8 (Cat. No. NBP2-22272, left panel ,NBP2-22273, middle panel and Cat. No. NBP2-22274, right panel) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB 100 μL Log in to see 7 to 9 Days
$559.35
Details

More Antibodies against PRPF8 Interaction Partners

Zebrafish PRP8 Pre-MRNA Processing Factor 8 Homolog (S. Cerevisiae) (PRPF8) interaction partners

  1. Within early haematopoiesis, myeloid differentiation is impaired, suggesting Prpf8 is required for haematopoietic development.

Human PRP8 Pre-MRNA Processing Factor 8 Homolog (S. Cerevisiae) (PRPF8) interaction partners

  1. Multiple genes contributing to the retinal dystrophy genotypes within a family were discovered using retinal gene-targeted next-generation sequencing. Families with noted examples of phenotypic variation or apparent non-penetrant individuals may offer a clue to suspect complex inheritance.

  2. Frame-shift mutations and nonconservative amino acid changes in PRPF8 typically cause severe clinical phenotypes. The conservative missense variant p.PRPF8-Arg2310Lys that is not altering the global charge of the C-terminal tail, and variants located at the basis of the C-terminal tail show milder clinical phenotypes, in accordance with functional data on PRPF8/SNRNP200 interactions in yeast.

  3. We show that PRP31, a component of U4 snRNP, is modified with K63-linked ubiquitin chains by the PRP19 complex and deubiquitinated by USP15 and its substrate targeting factor SART3. USP15SART3 makes a complex with USP4 and this ternary complex serves as a platform to deubiquitinate PRP31 and PRP3

  4. HSP90/R2TP chaperone system promotes the assembly of a key module of U5 snRNP while assuring the quality control of PRPF8. The proteomics data further reveal new interactions between R2TP and the tuberous sclerosis complex, pointing to a potential link between growth signals and the assembly of key cellular machines.

  5. influenza A virus upregulates cellular PRPF8 gene expression through viral NS1 protein and influenza virus polymerase basic protein 1 to increase virus production.

  6. Our findings exemplify the regulatory potential of changes in the core spliceosome machinery, which may be relevant to slow-onset human genetic diseases linked to PRPF8 deficiency

  7. Most importantly between Prp8 and nucleotides at the exon-intron junction.

  8. A mutation in a splicing factor PRPF8 that causes retinitis pigmentosa has a transcriptome-wide effect on mRNA splicing.

  9. Data suggest Enterovirus 3DPol (RNA-dependent RNA polymerase) enters nucleus via nuclear localization signal, targets pre-mRNA processing factor 8 (Prp8) to block pre-mRNA splicing/mRNA synthesis, and shuts off cellular transcription/translation.

  10. In the cytoplasm, Prp8 forms a precursor complex with U5 snRNA

  11. these data show how a Ski2-like RNA helicase Brr2 can be reversibly inhibited by a protein cofactor Prp8 that directly competes with RNA substrate binding.

  12. This is the first report of marked intrafamilial variability associated with mutations in the PRPF8 gene, including incomplete penetrance. PRPF8 mutations should be suspected in patients with autosomal dominant retinitis pigmentosa.

  13. RP-PRPF defects affect the stoichiometry of spliceosomal small nuclear RNAs. Depleting PRPF8 in human cell lines alters alternative splicing.

  14. Data screened retinitis pigmentosa patients for PRPF8 mutations and identified three new missense mutations, including the first documented mutation outside exon 42 and the first de novo mutation.

  15. Mutations in PRPC8 is associated with autosomal dominant retinitis pigmentosa

  16. mutations revealed a novel insertion and deletion in the last exon of a splicing factor gene, PRPF8.

  17. Nine mutations, six of which are novel, in the pre-mRNA splicing-factor genes PRPF3, PRPF8, and PRPF31, causing adRP have been identified in the Spanish population.

  18. The gene for apoptosis regulated protein 2 (ARP2) overexpressed in apoptosis induced prostate cancer cell line LNCaP was cloned.

  19. The PRPF8 gene mutation is associated with a mild phenotype in which cone function is partially preserved.

  20. The expanded Prp8 Jab1/MPN domain represents a pseudoenzyme converted into a protein-protein interaction platform and that dysfunction of this platform underlies Retinitis pigmentosa.

PRPF8 Antigen Profile

Protein Summary

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.

Gene names and symbols associated with PRPF8

  • pre-mRNA processing factor 8 (Prpf8) antibody
  • pre-mRNA processing factor 8 (prpf8) antibody
  • pre-mRNA processing factor 8 S homeolog (prpf8.S) antibody
  • pre-mRNA processing factor 8 (PRPF8) antibody
  • AU019467 antibody
  • D11Bwg0410e antibody
  • DBF3/PRP8 antibody
  • HPRP8 antibody
  • id:ibd1257 antibody
  • ik:tdsubc_2a9 antibody
  • im:7141966 antibody
  • prp-8 antibody
  • Prp8 antibody
  • PRPC8 antibody
  • RP13 antibody
  • Sfprp8l antibody
  • SNRNP220 antibody
  • tdsubc_2a9 antibody
  • wu:fb37c02 antibody
  • wu:fb73e06 antibody
  • xx:tdsubc_2a9 antibody
  • zgc:56504 antibody

Protein level used designations for PRPF8

PRP8 pre-mRNA processing factor 8 homolog , pre-mRNA-processing-splicing factor 8 , 220 kDa U5 snRNP-specific protein , PRP8 homolog , U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p , apoptosis-regulated protein 1 , apoptosis-regulated protein 2 , p220 , precursor mRNA processing protein , splicing factor Prp8

GENE ID SPECIES
287530 Rattus norvegicus
393951 Danio rerio
379945 Xenopus laevis
417559 Gallus gallus
480651 Canis lupus familiaris
507371 Bos taurus
10594 Homo sapiens
192159 Mus musculus
Selected quality suppliers for anti-PRPF8 (PRPF8) Antibodies
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