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Blocked agarose beads Accessory Reagents

Pubmed (1)
Catalog No. ABIN1082210
$90.00
Plus shipping costs $45.00
500 μL
local_shipping Shipping to: United States
Delivery in 5 to 6 Business Days
  • Application
    Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif), Separation (Sep)
    Purpose
    Blocked agarose beads as binding control for Dnmt1-Trap A, GFP-Trap A, RFP-Trap A
    Characteristics
    Antibodies - extremely powerful tools in biomedical research - are large complex molecules (~ 150 kDa) consisting of two heavy and two light chains. Due to their complex structure, the use of antibodies is often limited and hindered by batch-to-batch variations.

    Camelidae (camels, dromedaries, llamas and alpacas) possess functional antibodies devoid of light chains, so-called heavy chain antibodies (hcAbs). hcAbs recognize and bind their antigens via a single variable domain (VHH). These VHH domains are the smallest intact antigen binding fragments (~ 13 kDa).

    Nano-Traps are based on single domain antibody fragments (VHHs) derived from alpaca.
    Components
    Blocked agarose beads
  • Comment

    Bead size ~ 80 µm

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    20% EtOH
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Expiry Date
    12 months
  • Berk, Maitra, Dawdy, Shabanowitz, Hunt, Wilson: "O-Linked ?-N-acetylglucosamine (O-GlcNAc) regulates emerin binding to barrier to autointegration factor (BAF) in a chromatin- and lamin B-enriched "niche"." in: The Journal of biological chemistry, Vol. 288, Issue 42, pp. 30192-209, 2013 (PubMed).

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