Blocked agarose beads

Catalog No. ABIN1082210

Product Details

Application: Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif), Separation (Sep)

Purpose: Blocked agarose beads as binding control for Dnmt1-Trap A, GFP-Trap A, RFP-Trap A

Characteristics: Antibodies - extremely powerful tools in biomedical research - are large complex molecules (~ 150 kDa) consisting of two heavy and two light chains. Due to their complex structure, the use of antibodies is often limited and hindered by batch-to-batch variations.

Camelidae (camels, dromedaries, llamas and alpacas) possess functional antibodies devoid of light chains, so-called heavy chain antibodies (hcAbs). hcAbs recognize and bind their antigens via a single variable domain (VHH). These VHH domains are the smallest intact antigen binding fragments (~ 13 kDa).

Nano-Traps are based on single domain antibody fragments (VHHs) derived from alpaca.

Components: Blocked agarose beads

Application Details

Comment:

Bead size ~ 80 µm

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 20% EtOH

Handling Advice: Do not freeze.

Storage: 4 °C

Expiry Date: 12 months

References

Berk, Maitra, Dawdy, Shabanowitz, Hunt, Wilson: "O-Linked ?-N-acetylglucosamine (O-GlcNAc) regulates emerin binding to barrier to autointegration factor (BAF) in a chromatin- and lamin B-enriched "niche"." in: The Journal of biological chemistry, Vol. 288, Issue 42, pp. 30192-209, 2013 (PubMed).