- Antibody Type
- Recombinant Antibody
- Atto 594
- Fluorescence Microscopy (FM), Immunofluorescence (IF)
- With our Booster you reactivate, boost, stabilizate the signals of your fusion proteins.
- RFP-Booster efficiently highlights, enhancesand stabilizes monomeric red fluorescentproteins such as mRFP1, mCherry or mPlum but also mRuby
- Enhance, stabilize and reactivate your fl uorescent proteins
- RFP-Booster high specifi city for various monomeric red fl uorescent proteins derived from DsRed
- Coupled to bright and photostable chemical dyes from ATTO-TEC
- RFP-Trap® coupled to fluorescent dye ATTO 594
- Discover our best selling RFP Primary Antibody
- Application Notes
- For the immunofluorescence staining of RFP-fusion proteins in fixed cells
Booster are very small, highly specific GFP- or RFP-binding proteins covalently coupled to the superior fluorescent dyes from ATTO-TEC.
- Assay Procedure
- 1. Fixation: 4% paraformaldehyde (PFA) or 1:10 formalin (37% formaldehyde, 10-15% MetOH) in PBS, 10 min., RT.
- 2. Wash 3x with PBS containing 0.1% Tween 20 (PBST). Critical: do not let coverslips “dry”.
- 3. Permeabilisation: PBS containing 0.5% Triton X-100, 5 min., RT. Alternatively, permeabilise by incubating in 100% methanol for 5 min at -20°C.
- 4. Wash 2x with PBST.
- 5. Blocking: 4% BSA in PBST, 10 min, RT.
- 6. RFP-Booster incubation: dilute RFP-Booster 1:200 – 1:400 in blocking buffer and incubate 1 h, RT.
Note: For multiplexing protocols you can combine RFP-Booster with any other antibody.
- 7. Wash 3x 5-10 min in PBST.
- 8. If required, counterstain with DNA fluorescent dyes, e.g. DAPI.
- 9. Before mounting, coverslips can be very briefly rinsed in water to prevent salt crystals to form.
- 10. Mount in VectaShield (Vector Labs) or other mounting media with anti-fading agents and seal mounted coverslips with clear nail polish.
- For Research Use only
- 0.2 mg/ml
- PBS, 0.01% Sodium azide
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze. Protect from light.
- 4 °C
- Expiry Date
- 6 months
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- Alternative Name
- RFP ()
- Red fluorescent proteins (RFP) and variants thereof are widely used to study protein localization and dynamics in living cells. However, photo stability and quantum efficiency of RFP are not sufficient for Super-Resolution Microscopy (e.g. 3D-SIM or STED) of fixed samples. In addition, many cell biological methods such as BrdU-staining, EdU-Click-iT™ treatment or Fluorescent In Situ Hybridization result in disruption of the RFP signal.The RFP-Booster_Atto594, a specific RFP-binding protein coupled to the fluorescent dye ATTO 594, reactivates, boosts and stabilizes your RFP signal.