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The Spot-Tag® is a 12-amino acid epitope tag that is specifically recognized by monovalent Spot-Label, a 14.7 kDa small, high-affinity recombinant alpaca antibody fragment. The Spot-Tag system is useful for the purification of Spot-Tag fusion proteins and their analysis using immunoprecipitation, immunofluorescence, and super-resolution microscopy. Spot-Label can be used for Western blot, immunoprecipitation and ELISA analysis of Spot-Tag fusion proteins.