Spot-Label ATTO488 for Western blot, monovalent

Details for Product No. ABIN5775884, Supplier: Log in to see
Target Name (Antigen)
Reactivity
Tag
Host
Camelid (Camelidae)
Antibody Type
Recombinant Antibody
Conjugate
Atto 488
Application
Western Blotting (WB)
Options
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Purpose Recombinant alpaca single-domain antibody conjugated to ATTO488 for the fluorescence-based Western blot analysis of Spot-Tag fusion proteins.
Brand Spot-Tag®
Specificity Spot-Label binds to the Spot-Tag sequence PDRVRAVSHWSS both at the N-terminus or the C-terminus.
Characteristics The Spot-Tag® is a 12-amino acid epitope tag that is specifically recognized by a 14.7 kDa small, monovalent, high affinity recombinant alpaca antibody fragment. The Spot-Tag system is useful for the purification of Spot-Tag fusion proteins and their analysis using immunoprecipitation, immunofluorescence, and superresolution microscopy. For one-step Western blot analysis of Spot-Tag fusion proteins, we recommend the Spot-Label ATTO488. VHH (Nanobody), alpaca monoclonal
Purification Affinity-purified antibody fragment
Components ATTO488 conjugated Spot-Label for WB & IP, monovalent
Excitation Emission Excitation range 480 - 510 nm (λabs= 500 nm)
Emission range 520 - 560 nm (λfl= 520 nm)
Target Name (Antigen)
Target Type Tag
Background The Spot-Tag has an affinity-optimized 12 amino acid sequence to be strongly bind to the Spot-Nanobody. The inert tag can be bound to the N-terminal and to the C-terminal side of the protein of interest by PCR using modified primers or by using any of the Spot vectors offered. The expression of Spot-tagged proteins has been successfully tested in bacteria, yeast, mammalian cell lines, and insect cells so far.

Features:
- Small size
- PDRVRAVSHWSS
- 12 amino acid sequence
- 1.4 kDa
- pI: 10.4
- Hydrophilic
- Inert
- Linear
- Extinction coefficient: 5,500 L•mol-1•cm-1

Benefits:
- Easy to clone
- Works N-terminally and C-terminally
- Good expression
- Tested in bacteria, yeast, mammalian cell lines, and insect cells
Application Notes Western blot: recommended starting dilution 1:5000
The optimal dilution depends on the application and should be determined by the end user. A titration from 1:2500 up to 1:25,000 is recommended.
Comment

For Western blot (WB), the monovalent format of the Spot-Label is used: one anti-Spot-Tag VHH/Nanobody labelled with ATTO488.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Purified recombinant protein in PBS 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage RT,4 °C
Storage Comment Shipped at ambient temperature. Upon receipt store at 4°C. Do not freeze.
Expiry Date 12 months
Supplier Images
 image for Spot-Label ATTO488 for Western blot, monovalent (ABIN5775884) Spot-Label ATTO488 for Western blot, monovalent
 image for Spot-Label ATTO488 for Western blot, monovalent (ABIN5775884) Spot-Label ATTO488 for Western blot, monovalent (Image 2)
Western Blotting (WB) image for Spot-Label ATTO488 for Western blot, monovalent (ABIN5775884) Spot-Label ATTO488 for Western blot, monovalent (Image 3)