anti-Rat (Rattus) CD11b antibody for Immunohistochemistry (Frozen Sections)

Recommended CD11b Antibody (supplied by: Log in to see )

Antigen
Integrin alpha M (ITGAM) Antibodies
  • CD11b/CD18
  • CR3
  • CR3A
  • Cd11b
  • F730045J24Rik
  • Ly-40
  • MAC1
  • Mac-1
  • Mac-1a
  • CD11B
  • MAC-1
  • MAC1A
  • MO1A
  • SLEB6
  • C3bi
  • integrin subunit alpha M
  • integrin alpha M
  • integrin, alpha M (complement component 3 receptor 3 subunit)
  • integrin alpha-M
  • ITGAM
  • Itgam
  • LOC100351865
Epitope
Internal Region, AA 250-350
48
24
18
16
13
13
10
8
8
8
6
5
5
5
5
5
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
776
528
182
98
37
33
31
25
18
14
10
10
9
9
6
5
4
4
2
2
Host
Rabbit
545
424
179
17
5
2
Clonality
Polyclonal
Conjugate
This CD11b antibody is un-conjugated
146
128
100
68
20
19
18
17
17
14
14
13
12
12
11
9
7
7
7
7
6
6
6
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
938
274
224
200
187
161
93
90
88
62
21
20
13
13
8
8
5
5
4
4
3
3
2
2
1
1
1
1
1
1
1
Options
Supplier
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Catalog No. ABIN409930
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.834635 ABIN284103 FACS IHC (fro) Mouse IgG2a Log in to see OX-42 0
10 ABIN2688912 BR IHC (fro) FACS IF IP Mouse IgG2a, kappa Log in to see OX 9
10 ABIN1177299 BR IHC (fro) FACS IF IP Mouse IgG2a, kappa Log in to see OX-42 7
8.5 ABIN2478198 IHC (fro) FACS IF IP Mouse IgG2a Log in to see OX-42 4
5.5 ABIN2688913 IHC (f) IHC (fro) FACS IHC (zinc) Mouse IgG2a, kappa Log in to see OX 4
5.5 ABIN2478210 IHC (fro) FACS IF IP Mouse IgG2a Log in to see OX-42 4
5.5 ABIN2478205 IHC (fro) FACS Biotin Mouse IgG2a Log in to see OX-42 4
4 ABIN120414 Func FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see OX-42 4
4 ABIN120410 FACS IHC (fro) Biotin Mouse IgG2a Log in to see OX-42 3
1 ABIN120413 Func FACS IHC (fro) IHC (p) IP Mouse IgG2a Log in to see OX-42 4
1 ABIN120412 Func FACS IHC (fro) IF IHC (p) IP Mouse IgG2a Log in to see OX-42 4
1 ABIN213152 FACS IHC IHC (fro) Mouse IgG1 Log in to see ED7 0
1 ABIN182004 FACS IHC (fro) IP Mouse IgG2a Log in to see OX-42 4
1 ABIN225690 BR FACS IHC IHC (fro) IP Rat IgG2b Log in to see M1-70 0
1 ABIN600694 FACS IHC IHC (fro) Mouse IgG1 Log in to see 0
1 ABIN198550 FACS IHC IHC (fro) Biotin Mouse IgG2a Log in to see 0
1 ABIN600700 FACS IHC IHC (fro) IP Mouse IgG2a Log in to see 0
1 ABIN119464 FACS IHC (fro) Mouse IgG1 Log in to see ED7 4
1 ABIN2478200 Func IHC (fro) FACS Mouse IgG1 Log in to see ED7 4
1 ABIN206706 FACS IHC IHC (fro) Biotin Mouse IgG2a Log in to see OX-42 0

General

Antigen Integrin alpha M (ITGAM) Antibodies
Epitope Internal Region, AA 250-350
(48), (24), (18), (16), (13), (13), (10), (8), (8), (8), (6), (5), (5), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(776), (528), (182), (98), (37), (33), (31), (25), (18), (14), (10), (10), (9), (9), (6), (5), (4), (4), (2), (2)
Host Rabbit
(545), (424), (179), (17), (5), (2)
Clonality
Polyclonal
Conjugate This CD11b antibody is un-conjugated
(146), (128), (100), (68), (20), (19), (18), (17), (17), (14), (14), (13), (12), (12), (11), (9), (7), (7), (7), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(938), (274), (224), (200), (187), (161), (93), (90), (88), (62), (21), (20), (13), (13), (8), (8), (5), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 37 references available
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Product Details anti-CD11b Antibody

Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN409930) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region (within residues 250-350) of the mouse CD11b protein. [Swiss-Prot# P05555]
Plasmids, Primers & others

Target Details CD11b

Product Details anti-CD11b Antibody Application Details Handling References for anti-CD11b antibody (ABIN409930) Images back to top
Antigen
Alternative Name CD11b (ITGAM Antibody Abstract)
Background Gene Symbol: ITGAM
Molecular Weight Theoretical MW: 127 kDa
Gene ID 3684
UniProt P11215, P05555
Pathways Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-CD11b Antibody Target Details CD11b Handling References for anti-CD11b antibody (ABIN409930) Images back to top
Application Notes Western Blot 2 μg/mL, Simple Western 1:50, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry-Paraffin 1:400, Immunohistochemistry-FrozenThis CD11b antibody is useful for Western blot analysis, Immunocytochemistry, Flow Cytometry (PMID 21422470) and IHC-paraffin embedded sections. In Western blot a specific band is observed ~ 160 kDa and an apparant non-specific band is observed ~ 56 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Raw 264.7 cells. This antibody does not appear to work in human with Western blot. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 23980916) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for CD11b Antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry-Paraffin Embedded Sections Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-CD11b Antibody Target Details CD11b Application Details References for anti-CD11b antibody (ABIN409930) Images back to top
Format Liquid
Concentration 1.00 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-CD11b antibody (ABIN409930)

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling Images back to top
Product cited in:

Chi, Adini, Birsner, Bazinet, Akula, DAmato: "PR1P ameliorates neurodegeneration through activation of VEGF signaling pathway and remodeling of the extracellular environment." in: Neuropharmacology, Vol. 148, pp. 96-106, 2019 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)).

Schmidt, Hobbing, Donninger, Clark: "RASSF1A Deficiency Enhances RAS-Driven Lung Tumorigenesis." in: Cancer research, Vol. 78, Issue 10, pp. 2614-2623, 2018

Periyasamy, Thangaraj, Guo, Hu, Callen, Buch: "Epigenetic Promoter DNA Methylation of miR-124 Promotes HIV-1 Tat-Mediated Microglial Activation via MECP2-STAT3 Axis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 38, Issue 23, pp. 5367-5383, 2018

Xu, Xu, Ge, Tian, Zhao, Guo: "Anti-inflammatory effect of low molecular weight fucoidan from Saccharina japonica on atherosclerosis in apoE-knockout mice." in: International journal of biological macromolecules, Vol. 118, Issue Pt A, pp. 365-374, 2018

Dörsam, Seiwert, Foersch, Stroh, Nagel, Begaliew, Diehl, Kraus, McKeague, Minneker, Roukos, Reißig, Waisman, Moehler, Stier, Mangerich, Dantzer, Kaina, Fahrer: "PARP-1 protects against colorectal tumor induction, but promotes inflammation-driven colorectal tumor progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 17, pp. E4061-E4070, 2018 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).

Kimizuka, Katagiri, Locascio, Shigeta, Sasaki, Shibata, Morse, Sîrbulescu, Miyatake, Reeves, Suematsu, Gelfand, Brauns, Poznansky, Tsukada, Kashiwagi: "Brief Exposure of Skin to Near-Infrared Laser Modulates Mast Cell Function and Augments the Immune Response." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 201, Issue 12, pp. 3587-3603, 2018 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).

Anderton, Bandala-Sanchez, Simpson, Rickard, Ng, Di Rago, Hall, Vince, Silke, Liccardi, Feltham: "RIPK1 prevents TRADD-driven, but TNFR1 independent, apoptosis during development." in: Cell death and differentiation, 2018 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Nürnberg, Kociok, Brockmann, Lischke, Crespo-Garcia, Reichhart, Wolf, Baumgrass, Eming, Beer-Hammer, Joussen: "Dataset on the activation of Müller cells through macrophages upon hypoxia in the retina." in: Data in brief, Vol. 16, pp. 489-500, 2018 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Flores-Martínez, García-Núñez, Rojas, Cano: "Stabilization of HIF-2α impacts pancreas growth." in: Scientific reports, Vol. 8, Issue 1, pp. 13713, 2018 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Baranova, Souza, Bocharov, Vishnyakova, Hu, Vaisman, Amar, Chen, Remaley, Patterson, Yuen, Star, Eggerman: "Human SR-BII mediates SAA uptake and contributes to SAA pro-inflammatory signaling in vitro and in vivo." in: PLoS ONE, Vol. 12, Issue 4, pp. e0175824, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Li, Lu, Sheng, Zhu, Sun, Zhou, Yang, Xue, Chen, Tian, Du, Yan, Zhu, Xing, Li, Lin, Qiu, Su, Huang, Yan, Yin: "Hexokinase 2-dependent hyperglycolysis driving microglial activation contributes to ischemic brain injury." in: Journal of neurochemistry, Vol. 144, Issue 2, pp. 186-200, 2017 (Sample species: Rat (Rattus)).

Gao, Gülcüler, Golbach, Block, Zarbock, Martin-Villalba: "Endothelial cell-derived CD95 ligand serves as a chemokine in induction of neutrophil slow rolling and adhesion." in: eLife, Vol. 5, 2017

Kim, Kang, Choi, Joo, Kim, Song: "Prime-boost immunization by both DNA vaccine and oncolytic adenovirus expressing GM-CSF and shRNA of TGF-β2 induces anti-tumor immune activation." in: Oncotarget, Vol. 8, Issue 9, pp. 15858-15877, 2017

Algieri, Rodriguez-Nogales, Garrido-Mesa, Camuesco, Vezza, Garrido-Mesa, Utrilla, Rodriguez-Cabezas, Pischel, Galvez: "Intestinal anti-inflammatory activity of calcium pyruvate in the TNBS model of rat colitis: Comparison with ethyl pyruvate." in: Biochemical pharmacology, Vol. 103, pp. 53-63, 2016 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Makar, Nimmagadda, Singh, Lam, Mubariz, Judge, Trisler, Bever: "TrkB agonist, 7,8-dihydroxyflavone, reduces the clinical and pathological severity of a murine model of multiple sclerosis." in: Journal of neuroimmunology, Vol. 292, pp. 9-20, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Kim, Kim, Wu, Park, Ha, Kim, Lee, Lee, Kwon: "Extracellular Release of CD11b by TLR9 Stimulation in Macrophages." in: PLoS ONE, Vol. 11, Issue 3, pp. e0150677, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Xu, Meng, Erben, Wang, Glauben, Kühl, Wu, Ma, Hu, Wang, Sun, Jia, Wu, Chen, Siegmund, Qin: "Myeloid-derived suppressor cells promote B-cell production of IgA in a TNFR2-dependent manner." in: Cellular & molecular immunology, 2016

Yang, Scott, Monickaraj, Xu, Ardekani, Nitta, Cabrera, McGuire, Mohideen, Das, Ghosh: "Basement membrane stiffening promotes retinal endothelial activation associated with diabetes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 2, pp. 601-11, 2016

Rawat, Spector: "Development and characterization of a human microglia cell model of HIV-1 infection." in: Journal of neurovirology, Vol. 23, Issue 1, pp. 33-46, 2016

Ulasov, Borovjagin, Kaverina, Schroeder, Shah, Lin, Baryshnikov, Cobbs: "MT1-MMP silencing by an shRNA-armed glioma-targeted conditionally replicative adenovirus (CRAd) improves its anti-glioma efficacy in vitro and in vivo." in: Cancer letters, Vol. 365, Issue 2, pp. 240-50, 2015

Images

Product Details anti-CD11b Antibody Target Details CD11b Application Details Handling References for anti-CD11b antibody (ABIN409930) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody
Western Blotting (WB) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Western Blot: Integrin alpha M/CD11b Antibody [ABIN4099304] - Detection of CD11b in R...
Simple Western (SimWes) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Simple Western: Integrin alpha M/CD11b Antibody [ABIN4099304] - Simple Western lane v...
Immunofluorescence (IF) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Immunocytochemistry/Immunofluorescence: Integrin alpha M/CD11b Antibody [ABIN4099304]...
Immunofluorescence (IF) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Immunocytochemistry/Immunofluorescence: Integrin alpha M/CD11b Antibody [ABIN4099304]...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Immunohistochemistry-Frozen: CD11b Antibody - Blue: DAPI+ nuclei Red: CD11b Human lu...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Immunohistochemistry-Frozen: CD11b Antibody - Blue: DAPI+ nuclei. Red: CD11b. Lung c...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Integrin alpha M (ITGAM) (AA 250-350), (Internal Region) antibody (ABIN409930) Immunohistochemistry-Frozen: CD11b Antibody - Mouse spleen. Image from verified cust...