anti-Mouse (Murine) DDX58 antibody for Enzyme Immunoassay

Recommended DDX58 Antibody (supplied by: Log in to see )

Antigen
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) Antibodies
  • RIG-I
  • RIGI
  • RLR-1
  • 6430573D20Rik
  • C330021E21
  • RHIV-1
  • RIG-1
  • DExD/H-box helicase 58
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
  • DEXD/H-box helicase 58
  • DDX58
  • Ddx58
Epitope
C-Term
17
10
5
5
5
4
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
166
88
30
21
17
3
2
Host
Rabbit
112
49
9
6
Clonality
Polyclonal
Conjugate
This DDX58 antibody is un-conjugated
12
8
6
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
131
58
47
41
27
13
11
7
7
4
2
2
1
Supplier
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Catalog No. ABIN357193
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5555173 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) Antibodies
Epitope C-Term
(17), (10), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(166), (88), (30), (21), (17), (3), (2)
Host Rabbit
(112), (49), (9), (6)
Clonality
Polyclonal
Conjugate This DDX58 antibody is un-conjugated
(12), (8), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(131), (58), (47), (41), (27), (13), (11), (7), (7), (4), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-DDX58 Antibody

Target Details DDX58 Application Details Handling References for anti-DDX58 antibody (ABIN357193) Images
Specificity This antibody detects RIG-I at C-term.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human RIG-I.
Isotype Ig Fraction

Target Details DDX58

Product Details anti-DDX58 Antibody Application Details Handling References for anti-DDX58 antibody (ABIN357193) Images back to top
Antigen
Alternative Name DDX58 (DDX58 Antibody Abstract)
Background DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. RIG-I contains RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, RIG-I triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). This protein is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.Synonyms: DEAD box protein 58, DKFZp434J1111, DKFZp686N19181, FLJ13599, Probable ATP-dependent RNA helicase DDX58, RIG-1, RIG-I, Retinoic acid-inducible gene 1 protein
Molecular Weight 106600 Da
Gene ID 23586, 9606
UniProt O95786
Pathways Activation of Innate immune Response, Hepatitis C

Application Details

Product Details anti-DDX58 Antibody Target Details DDX58 Handling References for anti-DDX58 antibody (ABIN357193) Images back to top
Application Notes ELISA 1: 1,000. Western blot 1: 100 - 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details References for anti-DDX58 antibody (ABIN357193) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-DDX58 antibody (ABIN357193)

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details Handling Images back to top
Background publications

Li, Chen, Kato, Gale, Lemon: "Distinct poly(I-C) and virus-activated signaling pathways leading to interferon-beta production in hepatocytes." in: The Journal of biological chemistry, Vol. 280, Issue 17, pp. 16739-47, 2005

Breiman, Grandvaux, Lin, Ottone, Akira, Yoneyama, Fujita, Hiscott, Meurs: "Inhibition of RIG-I-dependent signaling to the interferon pathway during hepatitis C virus expression and restoration of signaling by IKKepsilon." in: Journal of virology, Vol. 79, Issue 7, pp. 3969-78, 2005

Cui, Imaizumi, Yoshida, Borden, Satoh: "Retinoic acid-inducible gene-I is induced by interferon-gamma and regulates the expression of interferon-gamma stimulated gene 15 in MCF-7 cells." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 82, Issue 3, pp. 401-5, 2004

Imaizumi, Yagihashi, Hatakeyama, Yamashita, Ishikawa, Taima, Yoshida, Inoue, Fujita, Yagihashi, Satoh: "Expression of retinoic acid-inducible gene-I in vascular smooth muscle cells stimulated with interferon-gamma." in: Life sciences, Vol. 75, Issue 10, pp. 1171-80, 2004

Imaizumi, Hatakeyama, Yamashita, Yoshida, Ishikawa, Taima, Satoh, Mori, Wakabayashi: "Interferon-gamma induces retinoic acid-inducible gene-I in endothelial cells." in: Endothelium : journal of endothelial cell research, Vol. 11, Issue 3-4, pp. 169-73, 2004

Images

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details Handling References for anti-DDX58 antibody (ABIN357193) back to top
Supplier Images
 image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (C-Term) antibody (ABIN357193) anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (C-Term) antibody