anti-Mouse (Murine) DDX58 antibody for Immunohistochemistry

Recommended DDX58 Antibody (supplied by: Log in to see )

Antigen
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) Antibodies
  • RIG-I
  • RIGI
  • RLR-1
  • 6430573D20Rik
  • C330021E21
  • RHIV-1
  • RIG-1
  • DExD/H-box helicase 58
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
  • DEXD/H-box helicase 58
  • DDX58
  • Ddx58
Epitope
AA 201-713
17
9
5
5
5
4
4
3
3
3
3
3
3
3
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
178
90
32
21
17
2
2
Host
Mouse
123
50
9
5
Clonality
Monoclonal
Conjugate
This DDX58 antibody is un-conjugated
12
8
6
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
142
71
46
42
27
14
14
8
7
7
3
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN1169336
$ 500.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.673489 ABIN1860446 ICC IHC WB Rabbit IgG AA 1-211 Log in to see Polyclonal 0
8.673489 ABIN4350538 IHC IHC (p) SimWes WB Mouse IgG1 kappa Log in to see 2M6F10 2
8.673489 ABIN2434244 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
8.673489 ABIN1031791 IHC ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN4350536 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
1 ABIN3023649 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1169335 IP IHC WB Biotin Mouse IgG1 AA 201-713 Log in to see Alme-1 0
1 ABIN2619989 IHC IHC (p) IP WB Biotin Mouse IgG1 AA 201-713 Log in to see Alme-1 0
1 ABIN2619990 IHC IHC (p) IP WB Mouse IgG1 AA 201-713 Log in to see Alme-1 0
1 ABIN600176 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5688368 IHC IP WB Biotin Mouse IgG1 AA 201-713 Log in to see Alme-1 0
1 ABIN5688367 IHC IP WB Mouse IgG1 AA 201-713 Log in to see Alme-1 0
1 ABIN2878003 IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN200133 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1003083 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 4
1 ABIN5899218 IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN1585141 IHC ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN4241998 IHC IHC (p) WB DyLight 755 Mouse IgG1 kappa Log in to see 2M6F10 0
1 ABIN4242001 IHC IHC (p) WB DyLight 550 Mouse IgG1 kappa Log in to see 2M6F10 0
1 ABIN4242002 IHC IHC (p) WB DyLight 350 Mouse IgG1 kappa Log in to see 2M6F10 0

General

Antigen DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) Antibodies
Epitope AA 201-713
(17), (9), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(178), (90), (32), (21), (17), (2), (2)
Host Mouse
(123), (50), (9), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DDX58 antibody is un-conjugated
(12), (8), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(142), (71), (46), (42), (27), (14), (14), (8), (7), (7), (3), (2), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-DDX58 Antibody

Target Details DDX58 Application Details Handling References for anti-DDX58 antibody (ABIN1169336) Images
Specificity Recognizes human and mouse RIG-I.
Cross-Reactivity Human, Mouse (Murine)
Purification Purified from concentrated hybridoma tissue culture supernatant.
Purity >95 % (SDS-PAGE)
Immunogen Recombinant human RIG-I (aa 201-713).
Clone Alme-1
Isotype IgG1
Plasmids, Primers & others

Target Details DDX58

Product Details anti-DDX58 Antibody Application Details Handling References for anti-DDX58 antibody (ABIN1169336) Images back to top
Antigen
Alternative Name RIG-I (DDX58 Antibody Abstract)
Background RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5'-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-I recruits the CARD adaptor inducing IFN-beta (Cardif) in a CARD-CARD-dependent manner resulting in NF-kappaB and IRF3 activation.
UniProt O95786
Pathways Activation of Innate immune Response, Hepatitis C

Application Details

Product Details anti-DDX58 Antibody Target Details DDX58 Handling References for anti-DDX58 antibody (ABIN1169336) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details References for anti-DDX58 antibody (ABIN1169336) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer In PBS containing 10 % glycerol and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date 12 months

References for anti-DDX58 antibody (ABIN1169336)

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details Handling Images back to top
Product cited in:

Pythoud, Rothenberger, Martínez-Sobrido, de la Torre, Kunz: "Lymphocytic Choriomeningitis Virus Differentially Affects the Virus-Induced Type I Interferon Response and Mitochondrial Apoptosis Mediated by RIG-I/MAVS." in: Journal of virology, Vol. 89, Issue 12, pp. 6240-50, 2015

Weber, Gawanbacht, Habjan, Rang, Borner, Schmidt, Veitinger, Jacob, Devignot, Kochs, García-Sastre, Weber: "Incoming RNA virus nucleocapsids containing a 5'-triphosphorylated genome activate RIG-I and antiviral signaling." in: Cell host & microbe, Vol. 13, Issue 3, pp. 336-46, 2013

Gack, Nistal-Villán, Inn, García-Sastre, Jung: "Phosphorylation-mediated negative regulation of RIG-I antiviral activity." in: Journal of virology, Vol. 84, Issue 7, pp. 3220-9, 2010

Rebsamen, Meylan, Curran, Tschopp: "The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases." in: Cell death and differentiation, Vol. 15, Issue 11, pp. 1804-11, 2008

Images

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details Handling References for anti-DDX58 antibody (ABIN1169336) back to top
Supplier Images
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 201-713) antibody (ABIN1169336) Western blot analysis of human RIG-I in HeLa cells by using RIG-I, mAb (Alme-1) . Me...
Immunohistochemistry (IHC) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 201-713) antibody (ABIN1169336) Immunohistochemical staining of endogenous human RIG-I in different human tissues (pa...