anti-Mouse (Murine) DDX58 antibody for Western Blotting

Recommended DDX58 Antibody (supplied by: Log in to see )

Antigen
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) Antibodies
  • RIG-I
  • RIGI
  • RLR-1
  • 6430573D20Rik
  • C330021E21
  • RHIV-1
  • RIG-1
  • DExD/H-box helicase 58
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
  • DEXD/H-box helicase 58
  • DDX58
  • Ddx58
Reactivity
Mouse (Murine), Rat (Rattus), Human
171
75
32
2
2
2
Host
Rabbit
130
34
11
5
Clonality
Polyclonal
Conjugate
This DDX58 antibody is un-conjugated
12
7
5
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
132
56
46
30
27
17
17
9
8
7
3
1
Options
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Available images

Catalog No. ABIN3023649
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
23.255114 ABIN1169336 IP IHC WB Mouse IgG1 AA 201-713 Log in to see Alme-1 4
19.726997 ABIN388602 WB Rabbit Ig Fraction AA 894-925, C-Term Log in to see Polyclonal 9
19.726997 ABIN1860446 ICC IHC WB Rabbit IgG AA 1-211 Log in to see Polyclonal 0
19.726997 ABIN2923813 IF/ICC IHC IP WB Rabbit AA 1-211 Log in to see Polyclonal 0
18.226997 ABIN6673858 IHC WB Rabbit IgG Log in to see Polyclonal 2
12.028116 ABIN1169335 IP IHC WB Biotin Mouse IgG1 AA 201-713 Log in to see Alme-1 0
12.028116 ABIN6389912 ELISA WB Rabbit IgG Log in to see Polyclonal 0
10.726997 ABIN6266336 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
10.726997 ABIN2434244 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
9.226997 ABIN2476365 IHC (p) WB Rabbit IgG Log in to see Polyclonal 4
7.7269974 ABIN3030701 ELISA WB Rabbit Ig Fraction AA 894-925 Log in to see Polyclonal 0
7.7269974 ABIN5544929 WB Rabbit IgG Log in to see Polyclonal 0
7.7269974 ABIN2432609 ELISA WB Rabbit IgG Log in to see Polyclonal 0
7.7269974 ABIN1031791 IHC ELISA WB Rabbit Log in to see Polyclonal 0
7 ABIN730663 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
5.5 ABIN1003083 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 4
4.7269974 ABIN5518906 WB Rabbit IgG AA 871-925 Log in to see Polyclonal 0
4.7269974 ABIN6567946 WB Rabbit IgG Log in to see Polyclonal 0
4.7269974 ABIN5899217 WB Rabbit IgG Log in to see Polyclonal 0
4.7269974 ABIN4905007 WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) Antibodies
Reactivity Mouse (Murine), Rat (Rattus), Human
(171), (75), (32), (2), (2), (2)
Host Rabbit
(130), (34), (11), (5)
Clonality
Polyclonal
Conjugate This DDX58 antibody is un-conjugated
(12), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(132), (56), (46), (30), (27), (17), (17), (9), (8), (7), (3), (1)
Pubmed 2 references available
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Product Details anti-DDX58 Antibody

Target Details DDX58 Application Details Handling References for anti-DDX58 antibody (ABIN3023649) Images
Purification Affinity purification
Immunogen A synthetic peptide of human DDX58
Isotype IgG
Plasmids, Primers & others

Target Details DDX58

Product Details anti-DDX58 Antibody Application Details Handling References for anti-DDX58 antibody (ABIN3023649) Images back to top
Antigen
Alternative Name DDX58 (DDX58 Antibody Abstract)
Background Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes. TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments, TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection. Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling. Rig-I binds both dsRNA and viral ssRNA that contains a 5-triphosphate end not seen in host RNA. Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses. The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-kappaB, TBK1/IKKepsilon, and IRF-3/IRF-7.
Synonyms: DDX58, DKFZp434J1111, DKFZp686N19181, FLJ13599, RIG-I
Gene ID 23586
UniProt O95786
Pathways Activation of Innate immune Response, Hepatitis C

Application Details

Product Details anti-DDX58 Antibody Target Details DDX58 Handling References for anti-DDX58 antibody (ABIN3023649) Images back to top
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details References for anti-DDX58 antibody (ABIN3023649) Images back to top
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C

References for anti-DDX58 antibody (ABIN3023649)

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details Handling Images back to top
Product cited in:

Zhu, Ye, Ashraf, Li, Chen, Song, Cao: "Transcriptional regulation of miR-15b by c-Rel and CREB in Japanese encephalitis virus infection." in: Scientific reports, Vol. 6, pp. 22581, 2017 Method employed by authors: Western Blotting (WB)

Cheng, Liao, Zhou, Peng, Chen, Yuan: "Amplified RLR signaling activation through an interferon-stimulated gene-endoplasmic reticulum stress-mitochondrial calcium uniporter protein loop." in: Scientific reports, Vol. 6, pp. 20158, 2016 Method employed by authors: Western Blotting (WB)

Images

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details Handling References for anti-DDX58 antibody (ABIN3023649) back to top
Supplier Images
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Western blot analysis of extracts of various cell lines, using DDX58 antibody.
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Western blot analysis of extracts of various cell lines, using DDX58 antibody.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Immunohistochemistry of paraffin-embedded human prostate using DDX58 antibody.
Immunofluorescence (IF) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Immunofluorescence analysis of HeLa cells using DDX58 antibody.