anti-Mouse (Murine) DDX58 antibody for Western Blotting

Recommended DDX58 Antibody (supplied by: Log in to see )

Antigen
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) Antibodies
  • RIG-I
  • RIGI
  • RLR-1
  • 6430573D20Rik
  • C330021E21
  • RHIV-1
  • RIG-1
  • DExD/H-box helicase 58
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
  • DEXD/H-box helicase 58
  • DDX58
  • Ddx58
Epitope
AA 894-925, C-Term
17
10
5
5
4
4
4
3
3
3
3
3
3
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
162
73
33
2
2
2
Host
Rabbit
123
32
11
5
Clonality
Polyclonal
Conjugate
This DDX58 antibody is un-conjugated
11
7
5
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
125
54
44
30
27
16
15
9
8
7
3
1
Options
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Catalog No. ABIN388602
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2075982 ABIN5518906 WB Rabbit IgG AA 871-925 Log in to see Polyclonal 0
3.2075982 ABIN1169336 IP IHC WB Mouse IgG1 AA 201-713 Log in to see Alme-1 4
3.2075982 ABIN1860446 ICC IHC WB Rabbit IgG AA 1-211 Log in to see Polyclonal 0
3.2075982 ABIN4350536 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
3.2075982 ABIN3023649 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.2075982 ABIN2476365 IHC (p) WB Rabbit IgG Log in to see Polyclonal 4
3.2075982 ABIN5965558 WB Rabbit IgG Log in to see Polyclonal 0
3.2075982 ABIN6266336 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
3.2075982 ABIN2923813 IF/ICC IHC IP WB Rabbit AA 1-211 Log in to see Polyclonal 0
3.2075982 ABIN5899217 WB Rabbit IgG Log in to see Polyclonal 0
3.2075982 ABIN3030701 ELISA WB Rabbit Ig Fraction AA 894-925 Log in to see Polyclonal 0
3.2075982 ABIN5544929 WB Rabbit IgG Log in to see Polyclonal 0
3.2075982 ABIN2434244 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
3.2075982 ABIN2432609 ELISA WB Rabbit IgG Log in to see Polyclonal 0
3.2075982 ABIN1031791 IHC ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN357193 EIA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 5
1 ABIN730663 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5555173 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1169335 IP IHC WB Biotin Mouse IgG1 AA 201-713 Log in to see Alme-1 0
1 ABIN2619989 IHC IHC (p) IP WB Biotin Mouse IgG1 AA 201-713 Log in to see Alme-1 0

General

Antigen DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) Antibodies
Epitope AA 894-925, C-Term
(17), (10), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(162), (73), (33), (2), (2), (2)
Host Rabbit
(123), (32), (11), (5)
Clonality (Clone)
Polyclonal   ( )
Conjugate This DDX58 antibody is un-conjugated
(11), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(125), (54), (44), (30), (27), (16), (15), (9), (8), (7), (3), (1)
Pubmed 9 references available
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Product Details anti-DDX58 Antibody

Target Details DDX58 Application Details Handling References for anti-DDX58 antibody (ABIN388602) Images
Specificity This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human RIG-I.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This RIG-I antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 894-925 AA from the C-terminal region of human RIG-I.
Clone RB8008
Isotype Ig Fraction
Plasmids, Primers & others

Target Details DDX58

Product Details anti-DDX58 Antibody Application Details Handling References for anti-DDX58 antibody (ABIN388602) Images back to top
Antigen
Alternative Name RIG-I (DDX58 Antibody Abstract)
Background Synonyms: Probable ATP-dependent RNA helicase DDX58, DEAD-box protein 58, Retinoic acid-inducible gene 1 protein, RIG-1, RIG-I, DDX58,
Molecular Weight 106600 DA
Gene ID 23586
UniProt O95786
Pathways Activation of Innate immune Response, Hepatitis C

Application Details

Product Details anti-DDX58 Antibody Target Details DDX58 Handling References for anti-DDX58 antibody (ABIN388602) Images back to top
Application Notes WB = 1:1000
Restrictions For Research Use only

Handling

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details References for anti-DDX58 antibody (ABIN388602) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-DDX58 antibody (ABIN388602)

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details Handling Images back to top
Product cited in:

Munday, Howell, Barr, Hiscox: "Proteomic analysis of mitochondria in respiratory epithelial cells infected with human respiratory syncytial virus and functional implications for virus and cell biology." in: The Journal of pharmacy and pharmacology, Vol. 67, Issue 3, pp. 300-18, 2015 Method employed by authors: Immunofluorescence (IF)

Huang, Kolokoltsova, Yun, Seregin, Ronca, Koma, Paessler: "Highly Pathogenic New World and Old World Human Arenaviruses Induce Distinct Interferon Responses in Human Cells." in: Journal of virology, Vol. 89, Issue 14, pp. 7079-88, 2015 Method employed by authors: Western Blotting (WB)

Crill, Furr-Rogers, Marriott: "RIG-I is required for VSV-induced cytokine production by murine glia and acts in combination with DAI to initiate responses to HSV-1." in: Glia, 2015 Method employed by authors: Western Blotting (WB)

Feng, Langereis, Lork, Nguyen, Hato, Lanke, Emdad, Bhoopathi, Fisher, Lloyd, van Kuppeveld: "Enterovirus 2Apro targets MDA5 and MAVS in infected cells." in: Journal of virology, Vol. 88, Issue 6, pp. 3369-78, 2014 Method employed by authors: Western Blotting (WB)

Huang, Kolokoltsova, Yun, Seregin, Poussard, Walker, Brasier, Zhao, Tian, de la Torre, Paessler: "Junín virus infection activates the type I interferon pathway in a RIG-I-dependent manner." in: PLoS neglected tropical diseases, Vol. 6, Issue 5, pp. e1659, 2012 Method employed by authors: Western Blotting (WB)

Furr, Moerdyk-Schauwecker, Grdzelishvili, Marriott: "RIG-I mediates nonsegmented negative-sense RNA virus-induced inflammatory immune responses of primary human astrocytes." in: Glia, Vol. 58, Issue 13, pp. 1620-9, 2010 Method employed by authors: Western Blotting (WB)

Barral, Sarkar, Fisher, Racaniello: "RIG-I is cleaved during picornavirus infection." in: Virology, Vol. 391, Issue 2, pp. 171-6, 2009 Method employed by authors: Western Blotting (WB)

Bao, Liu, Shan, Li, Garofalo, Casola: "Human metapneumovirus glycoprotein G inhibits innate immune responses." in: PLoS pathogens, Vol. 4, Issue 5, pp. e1000077, 2008 Method employed by authors: Western Blotting (WB)

Liao, Bao, Liu, Lai, Li, Garofalo, Casola: "Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling." in: The Journal of general virology, Vol. 89, Issue Pt 8, pp. 1978-86, 2008 Method employed by authors: Western Blotting (WB)

Images

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details Handling References for anti-DDX58 antibody (ABIN388602) back to top
Supplier Images
 image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 894-925), (C-Term) antibody (ABIN388602) 24-hour post infection immunoblots of whole cell lysates from primary murine microgli...
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 894-925), (C-Term) antibody (ABIN388602) Western blot analysis of lysates from THP-1 cell line, untreated or treated with LPS,...
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) (AA 894-925), (C-Term) antibody (ABIN388602) All lanes : Anti-DDX58 C-term K909 at 1:2000 dilution Lane 1: Hela whole cell lysate ...