anti-Plasmodium falciparum Heat Shock 60kDa Protein 1 (Chaperonin) antibody for Immunocytochemistry

Recommended Heat Shock 60kDa Protein 1 (Chaperonin) Antibody (supplied by: Log in to see )

Antigen
Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antibodies
  • CPN60
  • GROEL
  • HLD4
  • HSP-60
  • HSP60
  • HSP65
  • HuCHA60
  • SPG13
  • chaperonin
  • cpn60
  • groel
  • hld4
  • hsp60
  • hsp65
  • spg13
  • 60kDa
  • Hsp60
  • 12
  • BP5
  • CG12101
  • Cpn60
  • Dmel\\CG12101
  • Dmhsp60
  • G62
  • HSP60A
  • Hsp60A
  • IEF16
  • Mmp-P1
  • SSP 7506
  • d-hsp60
  • hsp60A
  • l(1)10Ac
  • l(1)BP5
  • l(1)G8
  • l(1)HM21
  • l(1)L12
  • l(1)dp025
  • cb863
  • fa04a05
  • fb22d10
  • fi27b05
  • id:ibd2197
  • sb:cb144
  • wu:fa04a05
  • wu:fb22d10
  • wu:fi04a12
  • wu:fi27b05
  • MIF4
  • MNA2
  • mopA
  • groL
  • crpA
  • Hspd1-30p
  • heat shock protein family D (Hsp60) member 1
  • heat shock protein family D (Hsp60) member 1 S homeolog
  • 60 kDa heat shock protein, mitochondrial
  • heat shock protein 1 (chaperonin)
  • Heat shock protein 60A
  • heat shock 60 protein 1
  • chaperone ATPase HSP60
  • molecular chaperone GroEL
  • thermosome subunit
  • chaperonin GroEL
  • mitochondrial chaperonin
  • heat shock protein family D member 1
  • HSPD1
  • hspd1.S
  • hspd1
  • LOC100414401
  • Hspd1
  • Hsp60A
  • HSP60
  • groEL
  • MMP_RS07785
  • groEl
  • LOC100136430
Reactivity
Bacteria, Plasmodium falciparum
842
575
558
392
347
329
320
306
284
272
195
182
134
121
115
104
78
60
40
39
37
37
36
35
23
21
20
20
18
18
18
17
17
6
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
572
342
10
Clonality
Polyclonal
Conjugate
Un-conjugated
35
28
27
27
23
22
17
12
12
12
12
12
12
12
12
12
12
11
11
11
11
11
10
10
9
9
9
8
8
8
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
774
441
411
378
318
275
232
183
85
63
42
41
8
7
6
6
2
2
1
1
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Catalog No. ABIN361856
$ 383.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2484792 ICC IF WB Atto 633 Rabbit Log in to see Polyclonal 0
1 ABIN2484789 ICC IF WB Atto 488 Rabbit Log in to see Polyclonal 0
1 ABIN2484794 ICC IF WB Atto 680 Rabbit Log in to see Polyclonal 0
1 ABIN2484793 ICC IF WB Atto 655 Rabbit Log in to see Polyclonal 0
1 ABIN2484798 ICC IF WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN2484800 ICC IF WB HRP Rabbit Log in to see Polyclonal 0
1 ABIN2484799 ICC IF WB FITC Rabbit Log in to see Polyclonal 0
1 ABIN2484803 ICC IF WB PE Rabbit Log in to see Polyclonal 0
1 ABIN2484804 ICC IF WB Streptavidin Rabbit Log in to see Polyclonal 0
1 ABIN2484788 ICC IF WB Atto 390 Rabbit Log in to see Polyclonal 0
1 ABIN2484796 ICC IF WB Alkaline Phosphatase (AP) Rabbit Log in to see Polyclonal 0
1 ABIN2484801 ICC IF WB PE-Atto 594 Rabbit Log in to see Polyclonal 0
1 ABIN2484797 ICC IF WB APC Rabbit Log in to see Polyclonal 0
1 ABIN2484790 ICC IF WB Atto 565 Rabbit Log in to see Polyclonal 0
1 ABIN2484791 ICC IF WB Atto 594 Rabbit Log in to see Polyclonal 0
1 ABIN2484795 ICC IF WB Atto 700 Rabbit Log in to see Polyclonal 0
1 ABIN2484802 ICC IF WB PerCP Rabbit Log in to see Polyclonal 0
1 ABIN4221876 ICC IF WB Rabbit Log in to see Polyclonal 0

General

Antigen Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) Antibodies
Reactivity Bacteria, Plasmodium falciparum
(842), (575), (558), (392), (347), (329), (320), (306), (284), (272), (195), (182), (134), (121), (115), (104), (78), (60), (40), (39), (37), (37), (36), (35), (23), (21), (20), (20), (18), (18), (18), (17), (17), (6), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(572), (342), (10)
Clonality
Polyclonal
Conjugate Un-conjugated
(35), (28), (27), (27), (23), (22), (17), (12), (12), (12), (12), (12), (12), (12), (12), (12), (12), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(774), (441), (411), (378), (318), (275), (232), (183), (85), (63), (42), (41), (8), (7), (6), (6), (2), (2), (1), (1)
Pubmed 10 references available
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Product details

Target Details Application Details Handling References Images
Specificity Detects ~ 60 kDa. Cross-reacts with E.coli HSP60, GroEl.
Purification Protein A Purified
Immunogen Recombinant full length PfHSP60

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name HSP60 (HSPD1 Antibody Abstract)
Background In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
NCBI Accession XM_001347402
UniProt P34940
Pathways Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product details Target Details Handling References Images back to top
Application Notes
  • WB (1:2000)
  • optimal dilutions for assays should be determined by the user.
Comment

0.9 μg/ml of SPC-185 was sufficient for detection of PfHSP60 in 20 μg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1.83 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References

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Background publications

Verda, Kim, Ikehara, Statkute, Bronesky, Petrenko, Oyama, He, Link, Vahanian, Burt: "Hematopoietic mixed chimerism derived from allogeneic embryonic stem cells prevents autoimmune diabetes mellitus in NOD mice." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 2, pp. 381-6, 2008

Lai, Zhuang, Zhang: "[Stability of implants placed in different bone types]" in: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology, Vol. 42, Issue 5, pp. 292-3, 2007

Deocaris, Kaul, Wadhwa: "On the brotherhood of the mitochondrial chaperones mortalin and heat shock protein 60." in: Cell stress & chaperones, Vol. 11, Issue 2, pp. 116-28, 2006

Gupta, Knowlton: "HSP60, Bax, apoptosis and the heart." in: Journal of cellular and molecular medicine, Vol. 9, Issue 1, pp. 51-8, 2005

Hartl, Hayer-Hartl: "Molecular chaperones in the cytosol: from nascent chain to folded protein." in: Science (New York, N.Y.), Vol. 295, Issue 5561, pp. 1852-8, 2002

Itoh, Komatsuda, Ohtani, Wakui, Imai, Sawada, Otaka, Ogura, Suzuki, Hamada: "Mammalian HSP60 is quickly sorted into the mitochondria under conditions of dehydration." in: European journal of biochemistry / FEBS, Vol. 269, Issue 23, pp. 5931-8, 2002

LaVerda, Kalayoglu, Byrne: "Chlamydial heat shock proteins and disease pathology: new paradigms for old problems?" in: Infectious diseases in obstetrics and gynecology, Vol. 7, Issue 1-2, pp. 64-71, 1999

Bukau, Horwich: "The Hsp70 and Hsp60 chaperone machines." in: Cell, Vol. 92, Issue 3, pp. 351-66, 1998

Hartl: "Molecular chaperones in cellular protein folding." in: Nature, Vol. 381, Issue 6583, pp. 571-9, 1996

Jindal, Dudani, Singh, Harley, Gupta: "Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigen." in: Molecular and cellular biology, Vol. 9, Issue 5, pp. 2279-83, 1989

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
 image for anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibody (ABIN361856) PfHsp60, malarial parasite lysate.