anti-Dog (Canine) Integrin beta 2 antibody for Flow Cytometry

Recommended Integrin beta 2 Antibody (supplied by: Log in to see )

Antigen
Integrin beta 2 (ITGB2) Antibodies
  • CD18
  • LAD
  • LCAMB
  • LFA-1
  • MAC-1
  • MF17
  • MFI7
  • 2E6
  • AI528527
  • Cd18
  • Lfa1
  • ITGB2
  • itgb2
  • itgb2-a
  • itgb2-b
  • integrin subunit beta 2
  • integrin beta 2
  • integrin subunit beta 2 S homeolog
  • ITGB2
  • Itgb2
  • itgb2.S
Reactivity
Dog (Canine), Human
470
207
117
38
37
21
20
13
5
4
3
3
3
3
3
2
1
1
Host
Rat
359
161
152
10
3
1
Clonality
Monoclonal
Conjugate
This Integrin beta 2 antibody is conjugated to PE
82
71
50
27
14
11
10
8
8
8
7
6
6
6
6
6
6
5
5
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS)
465
282
187
141
95
91
77
74
55
40
27
17
11
9
7
6
4
2
1
1
1
1
1
1
1
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Catalog No. ABIN118555
$ 456.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.941537 ABIN118551 FACS Biotin Rat IgG2b Log in to see YFC118-3 2
12.941537 ABIN118552 FACS FITC Rat IgG2b Log in to see YFC118-3 3
12.941537 ABIN118553 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 2
12.941537 ABIN2478872 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 4
12.941537 ABIN254640 FACS Biotin Rat IgG2b Log in to see YFC118-3 0
12.941537 ABIN4899848 FACS WB Goat IgG AA 24-702 Log in to see Polyclonal 0
12.941537 ABIN2478853 IHC (fro) FACS IF IP Mouse IgG1 Log in to see CA1-4E9 4
12.941537 ABIN4327005 FACS ICC IF IHC IHC (fro) IP Rat IgG2b Log in to see YFC118-3 0
1 ABIN319697 FACS PE Rat IgG2b Log in to see YFC118-3 3
1 ABIN319696 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 3
1 ABIN118554 FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 2
1 ABIN2478878 Func IHC (fro) FACS IP Rat IgG2b Log in to see YFC118-3 4
1 ABIN2478877 FACS FITC Rat IgG2b Log in to see YFC118-3 4
1 ABIN2478879 FACS PE Rat IgG2b Log in to see YFC118-3 4
1 ABIN600732 IHC (fro) FACS IP Rat IgG2b Log in to see 0
1 ABIN206735 BR FACS IHC (fro) IP Biotin Rat IgG2b Log in to see 0
1 ABIN576530 BR FACS IHC (fro) IP PE Rat IgG2b Log in to see 0
1 ABIN4327024 FACS APC Rat IgG2b Log in to see YFC118-3 0
1 ABIN206736 BR FACS IHC (fro) IP Rat IgG2b Log in to see YFC118-3 0
1 ABIN1820130 BR FACS Func IHC (fro) IP Rat IgG2b Log in to see YFC118-3 0

General

Antigen Integrin beta 2 (ITGB2) Antibodies
Reactivity Dog (Canine), Human
(470), (207), (117), (38), (37), (21), (20), (13), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1)
Host Rat
(359), (161), (152), (10), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Integrin beta 2 antibody is conjugated to PE
(82), (71), (50), (27), (14), (11), (10), (8), (8), (8), (7), (6), (6), (6), (6), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(465), (282), (187), (141), (95), (91), (77), (74), (55), (40), (27), (17), (11), (9), (7), (6), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Integrin beta 2 Antibody

Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN118555) Images
Specificity YFC118.3 was clustered at the Fourth International Workshop on Leucocyte Differentiation Antigen as recognising the CD18 antigen. The main cellular reactivity of antibody YFC 118.3 is a strong reactivity with Leucocytes (platelets negative). YFC118.3 blocks homotypic adhesion and other lymphocyte functions which involve the LFA-1 antigen.
Purification Affinity chromatography on Protein G
Immunogen Human neutrophils. Spleen cells from immunised LOU rats were fused with cells of the Y3/Ag.1.2.3 rat myeloma cell line.
Clone YFC118-3
Isotype IgG2b

Target Details Integrin beta 2

Product Details anti-Integrin beta 2 Antibody Application Details Handling References for anti-Integrin beta 2 antibody (ABIN118555) Images back to top
Antigen
Alternative Name CD18 / ITGB2 (ITGB2 Antibody Abstract)
Background The CD18 antigen is an integral membrane glycoprotein of 95kD molecular weight - known as the b chain, of the LFA-1 complex. The CD18 antigen is non-covalently linked to CD11a, b, c molecules. The CD18 Molecule is the receptor for ICAM-1 and is thus important for cell adhesion.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7
Gene ID 3689
NCBI Accession NP_000202
UniProt P05107
Pathways NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation

Application Details

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Handling References for anti-Integrin beta 2 antibody (ABIN118555) Images back to top
Application Notes Flow cytometry: Use 10 μL of neat antibody to label 100 μL whole blood or 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details References for anti-Integrin beta 2 antibody (ABIN118555) Images back to top
Reconstitution SM1076R: Restore with 1 mL distilled water. SM1076RT: Restore with 25 μL distilled water.
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store antibody and control at -20 °C.

References for anti-Integrin beta 2 antibody (ABIN118555)

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling Images back to top
Background publications

Chabanne, Marchal, Kaplanski, Fournel, Magnol, Monier, Rigal: "Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes." in: Tissue antigens, Vol. 43, Issue 3, pp. 202-5, 1994

Bindon, Hale, Waldmann: "Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies." in: European journal of immunology, Vol. 18, Issue 10, pp. 1507-14, 1988

Galfrè, Milstein, Wright: "Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG." in: Nature, Vol. 277, Issue 5692, pp. 131-3, 1979

Images

Product Details anti-Integrin beta 2 Antibody Target Details Integrin beta 2 Application Details Handling References for anti-Integrin beta 2 antibody (ABIN118555) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin beta 2 (ITGB2) antibody (PE) (ABIN118555) Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18:RPE