Bicaudal D Homolog 1 (Drosophila) (BICD1) (C-Term) antibody

Details for Product No. ABIN1001896
Antigen
  • BICD
  • B830009D06Rik
  • mKIAA4125
  • bicaudal D homolog 1 L homeolog
  • BICD cargo adaptor 1
  • bicaudal D homolog 1 (Drosophila)
  • bicd1.L
  • BICD1
  • Bicd1
Epitope
C-Term
7
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
20
12
5
1
1
1
1
1
1
Host
Rabbit
19
1
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
13
6
3
2
2
1
1
1
Options
Immunogen BICD1 antibody was raised in rabbits against a 15 amino acid peptide from near the carboxy terminus of human BICD1.
Blocking Peptide Blocking peptide for this product available: ABIN1003754
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Antigen
Alternative Name BICD1 (Bicaudal D homolog 1) (BICD1 Antibody Abstract)
Background BICD1 was initially identified as the human homolog to Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. BICD1 can bind to dynein-dynactin and co-localizes with Rab6A on the trans-Golgi network and cytoplasmic vesicles, suggesting BICD1 regulates COPI-independent Golgi-ER transport. BICD1 will also interact with Rab6B, a Rab6 isoform that is expressed primarily in brain. Live cell imaging showed bi-directional movement of Rab6B structures in SK-N-SH neurites, indicating that BICD1 is involved in the Rab6B regulation of retrograde cargo transport in neuronal cells. At least two isoforms of BICD1 are known to exist, this antibody will only recognize the larger isoform.
Pathways Ribonucleoprotein Complex Subunit Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, Maintenance of Protein Location
Application Notes BICD1 antibody can be used for detection of BICD1 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Wanschers, van de Vorstenbosch, Schlager, Splinter, Akhmanova, Hoogenraad, Wieringa, Fransen: "A role for the Rab6B Bicaudal-D1 interaction in retrograde transport in neuronal cells." in: Experimental cell research, Vol. 313, Issue 16, pp. 3408-20, 2007 (PubMed).

Matanis, Akhmanova, Wulf, Del Nery, Weide, Stepanova, Galjart, Grosveld, Goud, De Zeeuw, Barnekow, Hoogenraad: "Bicaudal-D regulates COPI-independent Golgi-ER transport by recruiting the dynein-dynactin motor complex." in: Nature cell biology, Vol. 4, Issue 12, pp. 986-92, 2002 (PubMed).

Baens, Marynen: "A human homologue (BICD1) of the Drosophila bicaudal-D gene." in: Genomics, Vol. 45, Issue 3, pp. 601-6, 1998 (PubMed).

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