DDX3X antibody (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked) (N-Term)

Details for Product anti-DDX3X Antibody No. ABIN1002199
Antigen
  • DBX
  • DDX14
  • DDX3
  • HLP2
  • D1Pas1-rs2
  • Ddx3
  • Fin14
  • An3
  • pl10
  • DDX3X
  • DEAD-box helicase 3, X-linked
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked
  • DEAD-box helicase 3, X-linked L homeolog
  • DDX3X
  • Ddx3x
  • ddx3x.L
  • ddx3x
Alternatives
anti-Human DDX3X antibody for Immunoprecipitation
Epitope
N-Term
20
20
10
8
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
178
107
89
6
6
5
5
4
4
1
1
1
1
Host
Rabbit
159
19
Clonality
Polyclonal
Conjugate
This DDX3X antibody is un-conjugated
7
6
6
3
3
3
3
3
3
3
3
3
2
2
2
2
2
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
132
70
63
30
27
26
22
17
8
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5
2
2
1
1
Options
Immunogen DDX3 antibody was raised against a 16 amino acid peptide from near the amino terminus of human DDX3.
Blocking Peptide Blocking peptide for this product available: ABIN2862569
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Plasmids, Primers & others Plasmids, Primers & others DDX3X products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Dead-box Polypeptide 3 X-linked (DBX) (DDX3X Antibody Abstract)
Background DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.
Pathways Ribonucleoprotein Complex Subunit Organization, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Ribosome Assembly
Application Notes DDX3 antibody can be used for the detection of DDX3 by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user.) DDX3 antibody will detect both DDX3 and DBY. Antibody can also be used for immunocytochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Abdelhaleem: "RNA helicases: regulators of differentiation." in: Clinical biochemistry, Vol. 38, Issue 6, pp. 499-503, 2005 (PubMed).

Lahn, Page: "Functional coherence of the human Y chromosome." in: Science (New York, N.Y.), Vol. 278, Issue 5338, pp. 675-80, 1997 (PubMed).

Linder, Lasko, Ashburner, Leroy, Nielsen, Nishi, Schnier, Slonimski: "Birth of the D-E-A-D box." in: Nature, Vol. 337, Issue 6203, pp. 121-2, 1989 (PubMed).

Product cited in: Park, Lee, Kim, Song: "Assignment of a human putative RNA helicase gene, DDX3, to human X chromosome bands p11.3-->p11.23." in: Cytogenetics and cell genetics, Vol. 81, Issue 3-4, pp. 178-9, 1998 (PubMed).

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