SATB1 antibody (SATB Homeobox 1) (N-Term)

Details for Product anti-SATB1 Antibody No. ABIN1003121, Supplier: Log in to see
Antigen
  • 2610306G12Rik
  • AW413156
  • SATB1
  • sb:cb955
  • si:ch211-195k18.2
  • SATB homeobox 1
  • special AT-rich sequence binding protein 1
  • SATB homeobox 1a
  • SATB1
  • Satb1
  • satb1
  • satb1a
Alternatives
anti-Human SATB1 antibody for Western Blotting
Epitope
N-Term
20
15
15
13
7
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
123
59
35
7
5
3
3
2
2
1
1
Host
Rabbit
73
32
18
Clonality
Polyclonal
Conjugate
This SATB1 antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
104
52
40
32
27
15
14
11
7
4
3
2
2
2
Options
Supplier
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Immunogen SATB1 antibody was raised in rabbits against a 18 amino acid peptide near the amino terminus of the human SATB1.
Blocking Peptide Blocking peptide for this product available: ABIN1004191
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Plasmids, Primers & others Plasmids, Primers & others SATB1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name SATB1 (Special AT-rich sequence-binding protein 1) (SATB1 Antibody Abstract)
Background Human special AT-rich sequence-binding protein-1 (SATB1) is a nuclear matrix/scaffold-associated region DNA-binding protein, predominantly expressed in the thymus and pre-B cells. Like its homolog SATB2, SATB1 selectively binds double-stranded, special AT-rich DNA sequences in which one strand exclusively consists of well-mixed A, T, and C nucleotides. SATB1 contains a dimerization domain that shares similarity with the PDZ motif, identified as an indispensable element for high-affinity binding of SATB1 to DNA. SATB1 constitutes a functional nuclear architecture that has a 'cage-like' protein distribution surrounding heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex) and transcription factors recruitment. SATB1 functions as a 'genome organizer' essential for proper T-cell development. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic, and when ectopically expressed in non-metastatic cells, can induce invasive activity in vivo. At least two isoforms of SATB1 are known to exist.
Pathways Caspase Cascade in Apoptosis, Activated T Cell Proliferation
Application Notes SATB1 antibody can be used for detection of SATB1 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Purbey, Singh, Kumar, Mehta, Ganesh, Mitra, Galande: "PDZ domain-mediated dimerization and homeodomain-directed specificity are required for high-affinity DNA binding by SATB1." in: Nucleic acids research, Vol. 36, Issue 7, pp. 2107-22, 2008 (PubMed).

Szemes, Gyorgy, Paweletz, Dobi, Agoston: "Isolation and characterization of SATB2, a novel AT-rich DNA binding protein expressed in development- and cell-specific manner in the rat brain." in: Neurochemical research, Vol. 31, Issue 2, pp. 237-46, 2006 (PubMed).

Cai, Han, Kohwi-Shigematsu: "Tissue-specific nuclear architecture and gene expression regulated by SATB1." in: Nature genetics, Vol. 34, Issue 1, pp. 42-51, 2003 (PubMed).

Dickinson, Kohwi-Shigematsu: "Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential." in: Molecular and cellular biology, Vol. 15, Issue 1, pp. 456-65, 1995 (PubMed).

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