TRIM5 antibody (Tripartite Motif Containing 5) (C-Term)

Details for Product anti-TRIM5 Antibody No. ABIN1003377, Supplier: Log in to see
Antigen
  • TRIM5
  • RNF88
  • TRIM5alpha
  • EG667823
  • Gm8833
  • RGD1304579
  • tripartite motif containing 5
  • tripartite motif-containing 5
  • TRIM5
  • Trim5
Alternatives
anti-Human TRIM5 antibody for ELISA
Epitope
C-Term
29
20
11
9
7
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
140
7
6
1
1
1
Host
Rabbit
91
38
13
Clonality
Polyclonal
Conjugate
This TRIM5 antibody is un-conjugated
5
5
5
4
4
4
Application
Fluorescence Microscopy (FM), ELISA, Western Blotting (WB)
117
98
62
38
11
7
6
1
1
1
Options
Supplier
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Immunogen Rabbit polyclol TRIM5alpha antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of rhesus monkey TRIM5( (Genbank accession No. AAS48505).
Blocking Peptide Blocking peptide for this product available: ABIN1003907
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Plasmids, Primers & others Plasmids, Primers & others TRIM5 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name TRIM5alpha (Retroviral Restriction Factor) (TRIM5 Antibody Abstract)
Background TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha-variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5alpha variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5alpha acts as a natural barrier to cross-species retrovirus transmission.
Pathways Activation of Innate immune Response
Application Notes TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 1 to 2 µg/ml. (Optimal dilution should be determined by user.) A band at approximately 55 kDa can be detected. Antibody can also be used for immunofluorescence microscopy and ELISA and might be suited for other applications not tested so far. Anti-TRIM5 alpha is human specific but should presumably also react with rhesus protein.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Hatziioannou, Perez-Caballero, Yang, Cowan, Bieniasz: "Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 29, pp. 10774-9, 2004 (PubMed).

Stremlau, Owens, Perron, Kiessling, Autissier, Sodroski: "The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys." in: Nature, Vol. 427, Issue 6977, pp. 848-53, 2004 (PubMed).

Background publications Reymond, Meroni, Fantozzi, Merla, Cairo, Luzi, Riganelli, Zanaria, Messali, Cainarca, Guffanti, Minucci, Pelicci, Ballabio: "The tripartite motif family identifies cell compartments." in: The EMBO journal, Vol. 20, Issue 9, pp. 2140-51, 2001 (PubMed).

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