TRIM5 antibody (Tripartite Motif Containing 5)

Details for Product anti-TRIM5 Antibody No. ABIN1003380, Supplier: Log in to see
Antigen
  • TRIM5
  • RNF88
  • TRIM5alpha
  • EG667823
  • Gm8833
  • RGD1304579
  • tripartite motif containing 5
  • tripartite motif-containing 5
  • TRIM5
  • Trim5
Alternatives
anti-Human TRIM5 antibody for ELISA
Reactivity
Human
140
7
6
1
1
1
Host
Rabbit
91
38
13
Clonality
Polyclonal
Conjugate
This TRIM5 antibody is un-conjugated
5
5
5
4
4
4
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
117
98
61
38
11
7
6
1
1
1
1
Options
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Immunogen Rabbit polyclol TRIM5gamma antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy-terminus of human TRIM5gamma (Genbank accession No. NP_149083).
Blocking Peptide Blocking peptide for this product available: ABIN1004487
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Plasmids, Primers & others Plasmids, Primers & others TRIM5 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name TRIM5gamma (Retroviral Restriction Factor) (TRIM5 Antibody Abstract)
Background TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha-variant which also contains a carboxy-terminal B30.2 (SPRY) domain. While a function has not yet been assigned to TRIM5gamma, it is known that expression of TRIM5gamma variants from humans, rhesus monkeys, and African green monkeys enable resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. Furthermore, the TRIM5gamma isoform appears to serve as a scaffold for the assembly of endogenous BTBD1 and BTBD2 proteins and also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.
Pathways Activation of Innate immune Response
Application Notes TRIM5 gamma antibody can be used for detection of TRIM5 gamma by Western blot at 1 to 2 µg/ml. (Optimal dilution should be determined by user.) A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Hatziioannou, Perez-Caballero, Yang, Cowan, Bieniasz: "Retrovirus resistance factors Ref1 and Lv1 are species-specific variants of TRIM5alpha." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 29, pp. 10774-9, 2004 (PubMed).

Stremlau, Owens, Perron, Kiessling, Autissier, Sodroski: "The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys." in: Nature, Vol. 427, Issue 6977, pp. 848-53, 2004 (PubMed).

Xu, Yang, Moitra, Hashimoto, Rallabhandi, Kaul, Meroni, Jensen, Weissman, DArpa: "BTBD1 and BTBD2 colocalize to cytoplasmic bodies with the RBCC/tripartite motif protein, TRIM5delta." in: Experimental cell research, Vol. 288, Issue 1, pp. 84-93, 2003 (PubMed).

Background publications Reymond, Meroni, Fantozzi, Merla, Cairo, Luzi, Riganelli, Zanaria, Messali, Cainarca, Guffanti, Minucci, Pelicci, Ballabio: "The tripartite motif family identifies cell compartments." in: The EMBO journal, Vol. 20, Issue 9, pp. 2140-51, 2001 (PubMed).

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