CRLF2 antibody (Cytokine Receptor-Like Factor 2)

Details for Product anti-CRLF2 Antibody No. ABIN1003387
Antigen
  • CRLF2
  • CRL2
  • CRLF2Y
  • TSLPR
  • CRLM2
  • Ly114
  • Tpte2
  • Tslpr
  • Crl2
  • cytokine receptor like factor 2
  • cytokine receptor-like factor 2
  • CRLF2
  • Crlf2
Reactivity
Mouse (Murine)
75
32
23
Host
Rabbit
77
19
3
Clonality
Polyclonal
Conjugate
This CRLF2 antibody is un-conjugated
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
Application
ELISA, Western Blotting (WB)
54
29
26
15
15
6
5
2
2
1
Options
Immunogen TSLP receptor antibody was raised in rabbits against a 21 amino acid peptide from near the center of mouse TSLP receptor.
Blocking Peptide Blocking peptide for this product available: ABIN1004492
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Plasmids, Primers & others Plasmids, Primers & others CRLF2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name TSLP Receptor (CRLF2 Antibody Abstract)
Background Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. It is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Its receptor TSLP-R will bind TSLP in a low-affinity fashion in transfected cells, co-transfection with IL-7R-alpha cDNA results in high-affinity binding and a functional heteromeric complex. This heteromeric receptor requires stat5 for TSLP-mediated signal transduction and is inhibited by SOCS-1. The approximately 75kDa band seen in the immunoblot may be a post-translationally modified form of TSLP-R.
Application Notes TSLP receptor antibody can be used for detection of TSLP receptor by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Ziegler, Liu: "Thymic stromal lymphopoietin in normal and pathogenic T cell development and function." in: Nature immunology, Vol. 7, Issue 7, pp. 709-14, 2006 (PubMed).

Sims, Williams, Morrissey, Garka, Foxworthe, Price, Friend, Farr, Bedell, Jenkins, Copeland, Grabstein, Paxton: "Molecular cloning and biological characterization of a novel murine lymphoid growth factor." in: The Journal of experimental medicine, Vol. 192, Issue 5, pp. 671-80, 2000 (PubMed).

Levin, Koelling, Friend, Isaksen, Ziegler, Perlmutter, Farr: "Thymic stromal lymphopoietin: a cytokine that promotes the development of IgM+ B cells in vitro and signals via a novel mechanism." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 2, pp. 677-83, 1999 (PubMed).

Product cited in: Park, Martin, Garka, Gliniak, Di Santo, Muller, Largaespada, Copeland, Jenkins, Farr, Ziegler, Morrissey, Paxton, Sims: "Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor." in: The Journal of experimental medicine, Vol. 192, Issue 5, pp. 659-70, 2000 (PubMed).

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