UNC93B1 antibody (Unc-93 Homolog B1 (C. Elegans))

Details for Product anti-UNC93B1 Antibody No. ABIN1003411
Antigen
  • UNC93
  • IIAE1
  • UNC93B
  • Unc-93B1
  • Unc93b
  • unc-93 homolog B1, TLR signaling regulator
  • unc-93 homolog B1 (C. elegans)
  • UNC93B1
  • Unc93b1
Alternatives
anti-Rat (Rattus) UNC93B1 antibody for Western Blotting
Reactivity
Human, Mouse (Murine), Rat (Rattus)
32
19
18
5
3
2
2
2
1
Host
Rabbit
32
1
Clonality
Polyclonal
Conjugate
This UNC93B1 antibody is un-conjugated
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
25
9
7
6
2
1
1
1
Options
Immunogen Unc93b antibody was raised in rabbits against a 19 amino acid peptide from near the amino terminus of human Unc93b.
Blocking Peptide Blocking peptide for this product available: ABIN1004498
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Plasmids, Primers & others Plasmids, Primers & others UNC93B1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Unc93b (UNC93B1 Antibody Abstract)
Background The endoplasmic reticulum (ER) protein Unc93b, a human homolog of the C. elegans Unc93 gene, was initially identified by a forward genetic screen using N-ethyl-N-nitrosourea where a histidine-to-arginine substitution in Unc93b caused defects in Toll-like receptor (TLR) 3, 7 and 9 signaling. Unlike Unc93a, another homolog of the C. elegans Unc93 gene whose function is unknown, Unc93b specifically interacts with TLR3, 7 and 9, the histidine-to-arginine point mutation used to identify Unc93b abolishes this interaction. Mice carrying this point mutation are highly susceptible to infection with a number of viruses, indicating that Unc93b plays an important role in innate immunity. Multiple isoforms of Unc93a are known to exist. This antibody will not cross-react with Unc93a.
Pathways TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
Application Notes Unc93b antibody can be used for the detection of Unc93b by Western blot at 0.5 – 1 µg/ml. (Optimal dilution should be determined by user). Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Product cited in: Brinkmann, Spooner, Hoebe, Beutler, Ploegh, Kim: "The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling." in: The Journal of cell biology, Vol. 177, Issue 2, pp. 265-75, 2007 (PubMed).

Tabeta, Hoebe, Janssen, Du, Georgel, Crozat, Mudd, Mann, Sovath, Goode, Shamel, Herskovits, Portnoy, Cooke, Tarantino, Wiltshire, Steinberg, Grinstein, Beutler: "The Unc93b1 mutation 3d disrupts exogenous antigen presentation and signaling via Toll-like receptors 3, 7 and 9." in: Nature immunology, Vol. 7, Issue 2, pp. 156-64, 2006 (PubMed).

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