Cyclin O (CCNO) (Internal Region) antibody

Details for Product No. ABIN1003415
Antigen
  • RGD1565217
  • ccnu
  • udg2
  • ung2
  • CCNU
  • MGC131111
  • C86987
  • Ccnu
  • E130318K11
  • Ung2
  • UDG2
  • cyclin O
  • cyclin O L homeolog
  • Ccno
  • ccno
  • CCNO
  • ccno.L
Epitope
Internal Region
19
16
4
4
2
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
47
13
11
2
1
1
Host
Rabbit
47
1
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
Application
ELISA, Western Blotting (WB)
44
26
8
7
1
1
Options
Immunogen UNG2 antibody was raised against a 13 amino acid peptide from near the center of human UNG2.
Blocking Peptide Blocking peptide for this product available: ABIN1003990
Isotype IgG
Purification Affinity chromatography purified via peptide column.
Antigen
Alternative Name Uracil-DNA Glycosylase 2 (UDG2) (CCNO Antibody Abstract)
Background The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promotes and alternative splicing. UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from DNA that arise through either misincorporation during replication or cytosine deamination . UNG2 can also be bound by the HIV-1 integrase and incorporated into the virion particle, suggesting that it is required to remove uracils from the viral genome. As the intrinsic antiviral protein APOBEC3G generates numerous uracils in the HIV genome during its replication, it may be that the UNG2 contributes to the APOBEC3G-mediated loss of infectivity by generating abasic sites in the viral genome. This UNG2 antibody will not cross-react with UNG1.
Application Notes UNG2 antibody can be used for the detection of UNG2 by Western blot at 1 – 2 µg/ml. (Optimal dilution should be determined by user.) Antibody can also be used for ELISA and might be suited for other applications not tested so far.
Restrictions For Research Use only
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months
Background publications Fromme, Verdine: "Base excision repair." in: Advances in protein chemistry, Vol. 69, pp. 1-41, 2004 (PubMed).

Harris, Bishop, Sheehy, Craig, Petersen-Mahrt, Watt, Neuberger, Malim: "DNA deamination mediates innate immunity to retroviral infection." in: Cell, Vol. 113, Issue 6, pp. 803-9, 2003 (PubMed).

Krokan, Otterlei, Nilsen, Kavli, Skorpen, Andersen, Skjelbred, Akbari, Aas, Slupphaug: "Properties and functions of human uracil-DNA glycosylase from the UNG gene." in: Progress in nucleic acid research and molecular biology, Vol. 68, pp. 365-86, 2001 (PubMed).

Willetts, Rey, Agostini, Navarro, Baudat, Vigne, Sire: "DNA repair enzyme uracil DNA glycosylase is specifically incorporated into human immunodeficiency virus type 1 viral particles through a Vpr-independent mechanism." in: Journal of virology, Vol. 73, Issue 2, pp. 1682-8, 1999 (PubMed).