CHUK antibody (conserved Helix-Loop-Helix Ubiquitous Kinase) (C-Term)

Details for Product anti-CHUK Antibody No. ABIN100660
Antigen
Epitope
C-Term
41
38
15
15
15
14
9
8
8
8
7
7
7
7
6
6
6
5
5
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
324
156
130
11
10
9
7
6
5
5
4
2
Host
Rabbit
285
45
7
1
Clonality
Polyclonal
Conjugate
This CHUK antibody is un-conjugated
19
10
10
8
8
8
7
7
7
7
7
7
2
2
2
2
2
Application
Western Blotting (WB)
276
136
102
43
36
36
27
21
18
11
4
3
2
2
2
1
Options
Immunogen IKK a peptide corresponding to the highly conserved of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Characteristics Concentration Definition: by Refractometry
Purification Antiserum
Sterility Sterile filtered
Antigen
Alternative Name IKK alpha (CHUK Antibody Abstract)
Background NFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death.  In the cytoplasm NFkB is negatively modulated by the inhibitory proteins IkB.  In turn IkB is phosphorylated by a cellular kinase complex called IKK.  IKK is a heterodimer composed of two kinases: IKK-a and IKK-b that phosphorylate IkB leading to its degradation and the resulting translocation of NFkB to the nucleus.  IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF- a, endotoxins and overexpression of cellular kinases like MEKK1.  Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.
Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha I-kappa-B kinase alpha IkBKA IKK-alpha IKK-A IkappaB kinase EC=2.7.11.10
Gene ID 1147
UniProt O15111
Pathways PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
Application Notes This product was assayed by immunoblot and found to be reactive against IKK a at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) Anti-IKK a is suitable for the detection by immunoblot of human, mouse and rat IKK a showing an 85 kDa band.  This product was assayed by immunoblot and found to be reactive against IKK a at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) Anti-IKK a is suitable for the detection by immunoblot of human, mouse and rat IKK a showing an 85 kDa band. 
Restrictions For Research Use only
Format Liquid
Concentration 80 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (C-Term) antibody (ABIN100660) Anti-IKKa antibody was diluted 1:500 to detect IKKa in human kidney tissue. Tissue wa...