Goat anti-Human IgA (Heavy Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN100717

Product Details

Target: IgA

Binding Specificity: Heavy Chain

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Cross-Reactivity: Human

Characteristics: Anti-Human IgA alpha heavy chain antibody generated in goat detects specifically Human IgA alpha heavy chain.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Anti-Human IgA alpha heavy chain antibody was produced by repeated immunization with Human IgA alpha heavy chain in goat.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 10-20

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-HUMAN IgA (alpha chain) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

ELISA Dilution: 1:60,000 - 1:600,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

References

Yu, Pollock, Duval, Lewis, Joseph, Meade, Cavacini: "Neutralization of HIV by milk expressed antibody." in: Journal of acquired immune deficiency syndromes (1999), Vol. 62, Issue 1, pp. 10-6, (2013) (PubMed).

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody

Background:

Synonyms: goat anti-Human IgA (alpha chain) biotin conjugated Antibody, goat anti-Human IgA alpha Antibody biotin conjugation

Background: Anti-Human IgA Biotin Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.