Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Bovine IgG Secondary Antibodies are designed for Western Blotting, ELISA and Immunohistochemistry. Biotin Conjugated Secondary antibodies can also be used for a variety of other applications such as Assay Development, Flow Cytometry and in situ hybridization. This product has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:11,000 to 1:45,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000