Goat anti-Donkey IgG (Fc Region) Antibody
-
- Target See all IgG products
- IgG
-
Binding Specificity
- Fc Region
-
Reactivity
- Donkey
-
Host
- Goat
-
Clonality
- Polyclonal
-
Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- IgG F(c)
- Cross-Reactivity
- Donkey
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Donkey IgG, Donkey IgG F(c) and Donkey Serum. No reaction was observed against Donkey IgG F(ab).
- Immunogen
-
Immunogen: Donkey IgG F(c) fragment
- Isotype
- IgG
-
-
- Application Notes
-
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Donkey IgG F(c) antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 5.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Expiry Date
- 12 months
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
-
Synonyms: goat anti-Donkey IgG F(c) fragment Antibody, goat anti-Donkey IgG Fc fragment Antibody, goat anti Donkey IgG Fc
Background: Anti-Donkey IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of donkey IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
-