Rabbit anti-Golden Syrian Hamster IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN101369

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Golden Syrian Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: IgG (H&L)

Characteristics: Anti-Golden Syrian Hamster IgG whole molecule antibody generated in rabbit detects specifically Golden Syrian Hamster IgG whole molecule. This secondary antibody anti-Golden Syrian Hamster IgG is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Golden Syrian Hamster IgG Anitbody is ideal for investigators in Cancer, Immunology, and Microbiology research.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: immunoaffinity chromatography using Hamster IgG coupled to agarose

Immunogen:

Immunogen: Hamster IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Golden Syrian Hamster IgG has been assayed against 1.0 μg of Hamster IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for Anti-Golden Syrian Hamster IgG.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: RT,4 °C,-20 °C

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Golden Syrian Hamster IgG (H&L) Antibody, Rabbit-a-Golden Syrian Hamster IgG, Golden Syrian Hamster IgG in Rabbit, Rabbit IgG Secondary Antibody, rabbit anti-hamster IgG antibody

Background: Anti-Golden Syrian Hamster IgG Biotin Antibody generated in rabbit detects Golden Syrian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Golden Syrian Hamster IgG Anitbody is ideal for investigators in Cancer, Immunology, and Microbiology research.