Goat anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody - Preadsorbed

Catalog No. ABIN101378

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Armenian Hamster

Host: Goat

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Specificity: IgG (H&L)

Cross-Reactivity: Armenian Hamster

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Armenian Hamster IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

ChIP Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:20,000 - 1:40,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 12 months

References

Köchl, Thelen, Vanes, Brazão, Fountain, Xie, Huang, Lyck, Stein, Tybulewicz: "WNK1 kinase balances T cell adhesion versus migration in vivo." in: Nature immunology, Vol. 17, Issue 9, pp. 1075-83, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: goat anti-Armenian Hamster IgG Antibody, goat anti-Hamster IgG Antibody

Background: Anti-Armenian Hamster IgG Antibody generated in goat detects Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.