Goat anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody - Preadsorbed

Details for Product No. ABIN101378
Antigen
Epitope
Heavy & Light Chain
1569
134
100
56
34
33
25
6
6
6
6
5
4
4
4
3
2
1
1
1
Reactivity
Armenian Hamster
355
342
336
233
232
122
113
87
80
73
72
65
61
57
54
49
43
31
26
15
9
9
7
5
4
4
4
4
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
845
799
335
169
93
84
23
22
13
5
4
4
4
3
1
1
1
1
Clonality
Polyclonal
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
Options
Immunogen

Immunogen: Armenian Hamster IgG whole molecule

Isotype IgG
Specificity IgG (H&L)
Cross-Reactivity Armenian Hamster
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Preadsorption: Solid phase absorption
Sterility Sterile filtered
Antigen
Background

Synonyms: goat anti-Armenian Hamster IgG Antibody, goat anti-Hamster IgG Antibody

Background: Anti-Armenian Hamster IgG Antibody generated in goat detects Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

ChIP Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:20,000 - 1:40,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions For Research Use only
Format Liquid
Concentration 2.1 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Expiry Date 12 months
Product cited in: Köchl, Thelen, Vanes, Brazão, Fountain, Xie, Huang, Lyck, Stein, Tybulewicz: "WNK1 kinase balances T cell adhesion versus migration in vivo." in: Nature immunology, Vol. 17, Issue 9, pp. 1075-83, 2017 (PubMed).