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Goat anti-Mouse IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB, FACS, FLISA, FM, IP, LF Polyclonal IgG unconjugated
Catalog No. ABIN101738
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 691
    • 672
    • 559
    • 452
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 95
    • 95
    • 92
    • 62
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 1717
    • 1474
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Goat
    Clonality
    • 3346
    • 164
    • 1
    Polyclonal
    Application
    • 1836
    • 1788
    • 1786
    • 1498
    • 962
    • 933
    • 912
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 49
    • 49
    • 32
    • 27
    • 25
    • 21
    • 16
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Immunoprecipitation (IP), Lateral Flow (LF)
    Specificity
    IgG (H&L)
    Cross-Reactivity
    Mouse (Murine)
    Characteristics
    Anti-Mouse IgG whole molecule antibody generated in goat detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Mouse IgG affinity purified secondary antibody is generated in goat detects specifically Mouse IgG whole molecule. This anti-Mouse IgG secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user.

    Immunoprecipitation Dilution: Yes

    FLISA Dilution: Yes

    ELISA Dilution: 1:25,000

    Flow Cytometry Dilution: Yes

    Western Blot Dilution: 1:3,000 - 1:15,000

    IF Microscopy Dilution: Yes

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: Goat anti-Mouse IgG Secondary Antibody, Mouse Secondary Antibody, GAM Antibody, Gt-a-Ms antibody, anti-mouse secondaries

    Background: Anti-Mouse IgG Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This unconjugated secondary antibody has been validated and optimized yielding good sensitivity and reproducible results with Rockland's primary antibodies.

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