Sheep anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN101802

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Sheep

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)

Specificity: IgG (H&L)

Characteristics: Anti-Mouse IgG whole molecule antibody generated in sheep detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in sheep.

Isotype: IgG

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Comment:

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Gentamicin sulfate

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Meade, Latorre-Margalef, Stallknecht, Krammer: "Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards." in: Emerging microbes & infections, Vol. 6, Issue 12, pp. e110, (2018) (PubMed).

Duehr, Wohlbold, Oestereich, Chromikova, Amanat, Rajendran, Gomez-Medina, Mena, tenOever, García-Sastre, Basler, Munoz-Fontela, Krammer: "Novel Cross-Reactive Monoclonal Antibodies against Ebolavirus Glycoproteins Show Protection in a Murine Challenge Model." in: Journal of virology, Vol. 91, Issue 16, (2017) (PubMed).

Leung, Cuddy, Wang, Rommens, Glogauer: "Sbds is required for Rac2-mediated monocyte migration and signaling downstream of RANK during osteoclastogenesis." in: Blood, Vol. 117, Issue 6, pp. 2044-53, (2011) (PubMed).

Leung, Wang, Cuddy, Sun, Magalhaes, Grynpas, Glogauer: "Filamin A regulates monocyte migration through Rho small GTPases during osteoclastogenesis." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 25, Issue 5, pp. 1077-91, (2010) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Synonyms: Mouse Antibody, Gt-a-Mouse IgA, Mouse Antibody in Goat, Mouse Secondary Antibody