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Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, WB Polyclonal IgG unconjugated
Catalog No. ABIN101951
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 692
    • 672
    • 557
    • 451
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 95
    • 95
    • 92
    • 62
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rabbit
    Host
    • 1716
    • 1474
    • 536
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Donkey
    Clonality
    • 3346
    • 164
    • 1
    Polyclonal
    Application
    • 1836
    • 1789
    • 1785
    • 1498
    • 960
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB)
    Specificity
    IgG (H&L)
    Characteristics
    Anti-Rabbit IgG antibody generated in donkey detects specifically rabbit IgG. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen
    Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
    Isotype
    IgG
  • Application Notes
    Anti-Rabbit IgG (H&l) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Storage
    4 °C
  • He, Qiu, Dai, Fang, Sun, Gong, Ding, Sun: "MicroRNA-351 inhibits denervation-induced muscle atrophy by targeting TRAF6." in: Experimental and therapeutic medicine, Vol. 12, Issue 6, pp. 4029-4034, (2016) (PubMed).

    Hua, Han, Zhou, Kim, Shi, Abeysirigunawardena, Jain, Singh, Aggarwal, Woodson, Ha: "An improved surface passivation method for single-molecule studies." in: Nature methods, Vol. 11, Issue 12, pp. 1233-6, (2015) (PubMed).

  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Synonyms: Rabbit Antibody, Dk-a-Rabbit IgG, Rabbit Antibody in Donkey, Rabbit Secondary Antibody.
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