Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

Details for Product No. ABIN102010, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
6235
2031
1776
1135
782
648
372
305
34
34
31
15
12
12
12
12
11
7
3
3
1
1
1
1
1
1
1
Reactivity
Rabbit
3081
2914
2380
1662
1509
786
737
489
476
411
401
395
342
298
271
160
159
111
55
34
30
26
16
15
12
10
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Host
Goat
7133
5286
1973
1077
507
362
88
84
51
35
23
15
8
5
3
1
1
Clonality
Polyclonal
Conjugate
HRP
2226
1823
1751
1266
794
718
430
343
235
147
145
140
140
116
113
112
102
98
94
93
92
91
89
84
81
80
78
75
73
72
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
48
43
41
37
32
31
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
12
11
11
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunoassay (IA)
Options
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Immunogen Rabbit IgG whole molecule
Isotype IgG
Specificity IgG (H&L)
Characteristics Anti Rabbit HRP Conjugated Secondary Antibodies are ideal for Western Blotting, Immunohistochemistry, ELISA as well as other anibody detection methods.
Concentration Definition: by UV absorbance at 280 nm
Antigen
Background Synonyms: goat anti rabbit conjugated secondary antibody
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Restrictions For Research Use only
Format Lyophilized
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Supplier Images
 image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN102010) Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)
Dot Blot (DB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN102010) secondary antibodies detect rabbit primary antibodies in a variety of platforms. Show...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN102010) HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in ABIN92561...
Western Blotting (WB) image for Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN102010) Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs,...
Product cited in: Grundmann, Merten, Malfacini, Inoue, Preis, Simon, Rüttiger, Ziegler, Benkel, Schmitt, Ishida, Müller, Reher, Kawakami, Inoue, Rick, Kühl, Imhof, Aoki, König, Hoffmann, Gomeza, Wess, Kostenis: "Lack of beta-arrestin signaling in the absence of active G proteins." in: Nature communications, Vol. 9, Issue 1, pp. 341, 2018 (PubMed).

Schmitz, Schrage, Gaffal, Charpentier, Wiest, Hiltensperger, Morschel, Hennen, Häußler, Horn, Wenzel, Grundmann, Büllesbach, Schröder, Brewitz, Schmidt, Gomeza, Galés, Fleischmann, Tüting, Imhof et al.: "A cell-permeable inhibitor to trap Gαq proteins in the empty pocket conformation. ..." in: Chemistry & biology, Vol. 21, Issue 7, pp. 890-902, 2015 (PubMed).

Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).