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Rabbit anti-Human IgM (Fc5mu Region) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
Catalog No. ABIN102642
  • Target See all IgM products
    IgM
    Binding Specificity
    • 141
    • 118
    • 87
    • 41
    • 16
    • 8
    • 7
    • 1
    • 1
    Fc5mu Region
    Reactivity
    • 188
    • 130
    • 65
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    Human
    Host
    • 321
    • 187
    • 73
    • 15
    • 14
    • 5
    • 4
    • 1
    • 1
    Rabbit
    Clonality
    • 495
    • 78
    Polyclonal
    Conjugate
    • 119
    • 77
    • 76
    • 54
    • 36
    • 32
    • 24
    • 15
    • 12
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Biotin
    Application
    • 332
    • 249
    • 238
    • 208
    • 143
    • 139
    • 64
    • 63
    • 47
    • 47
    • 46
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    IgM Fc5µ
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Human IgM Fc5mu fragment

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:40,000 to 1:190,000 is suggested for this product.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.5 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Aliquot to Avoid repeated freezing and thawing.
    Storage
    RT,4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
    Background

    Synonyms: Rabbit Anti-Human IgM (Fc5u) Antibody Biotin Conjugated, Rabbit Anti Human IgM Fc5u Antibody Biotin Conjugated

    Background: Anti-Human IgM Fc5μ Biotin antibody generated in rabbit specifically detects Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Due to this large size, IgM is typically isolated to the serum. Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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