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Rabbit anti-Rat IgM (Heavy Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
Catalog No. ABIN102711
  • Target See all IgM products
    IgM
    Binding Specificity
    • 141
    • 117
    • 87
    • 41
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy Chain
    Reactivity
    • 189
    • 130
    • 64
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    Rat
    Host
    • 321
    • 187
    • 73
    • 15
    • 14
    • 5
    • 4
    • 1
    • 1
    Rabbit
    Clonality
    • 495
    • 78
    Polyclonal
    Application
    • 332
    • 249
    • 238
    • 208
    • 143
    • 139
    • 64
    • 63
    • 47
    • 47
    • 46
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    IgM µ chain
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Rat IgM whole molecule

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Rat IgM mu heavy chain antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

    ELISA Dilution: 1:50,000 - 1:250,000

    Western Blot Dilution: 1:5,000 - 1:25,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.4 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgM
    Abstract
    IgM Products
    Target Type
    Antibody
    Background

    Synonyms: Rabbit Anti Rat IgM (mu chain) Antibody, Rabbit Anti-Rat IgM mu chain Antibody

    Background: Anti-Rat IgM antibody generated in rabbit specifically detects rat IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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