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DNMT1 antibody (AA 620-950)

DNMT1 Reactivity: Human WB, IHC, IP, ChIP Host: Mouse Monoclonal 4G11-C7 unconjugated
Catalog No. ABIN1027706
  • Target See all DNMT1 Antibodies
    DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
    Binding Specificity
    • 27
    • 22
    • 16
    • 14
    • 12
    • 11
    • 10
    • 9
    • 8
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 620-950
    Reactivity
    • 203
    • 56
    • 46
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 156
    • 47
    • 1
    • 1
    Mouse
    Clonality
    • 144
    • 61
    Monoclonal
    Conjugate
    • 81
    • 17
    • 14
    • 12
    • 10
    • 10
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    This DNMT1 antibody is un-conjugated
    Application
    • 133
    • 68
    • 45
    • 41
    • 33
    • 31
    • 28
    • 18
    • 17
    • 15
    • 14
    • 14
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
    Specificity
    Detects ~180 kDa. It will cross-react with mouse DNMT1.
    Cross-Reactivity
    Fish, Human, Mouse, Zebrafish (Danio rerio)
    Purification
    Protein G Purified
    Immunogen
    Raised against a partial protein corresponding to amino acids 620-950 of human DNMT1
    Clone
    4G11-C7
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product DNMT1 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • IHC (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    2 μg/ml of ABIN1027706 was sufficient for detection of Dnmt1 in 10 μg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Target
    DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))
    Alternative Name
    DNMT1 (DNMT1 Products)
    Synonyms
    cb1075 antibody, cb983 antibody, dmnt1 antibody, fb05h01 antibody, mta antibody, wu:fb05h01 antibody, ADCADN antibody, AIM antibody, CXXC9 antibody, DNMT antibody, HSN1E antibody, MCMT antibody, DDM2 antibody, DECREASED DNA METHYLATION 2 antibody, DMT01 antibody, DMT1 antibody, DNA METHYLTRANSFERASE antibody, DNA METHYLTRANSFERASE 01 antibody, DNA METHYLTRANSFERASE 1 antibody, MET2 antibody, METHYLTRANSFERASE 2 antibody, METHYLTRANSFERASE I antibody, METI antibody, methyltransferase 1 antibody, Cxxc9 antibody, Dnmt antibody, Dnmt1o antibody, MTase antibody, Met-1 antibody, Met1 antibody, MommeD2 antibody, m.MmuI antibody, DNA methyltransferase 1 antibody, DNA (cytosine-5-)-methyltransferase 1 antibody, methyltransferase 1 antibody, DNA methyltransferase (cytosine-5) 1 antibody, DNMT1 antibody, dnmt1 antibody, Dnmt1 antibody, MET1 antibody
    Background
    Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).
    Gene ID
    1786
    NCBI Accession
    NP_001370
    UniProt
    P26358
    Pathways
    SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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