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IL-27 antibody

IL27 Reactivity: Mouse WB Host: Rat Monoclonal 3H12-F10 unconjugated
Catalog No. ABIN1043836
  • Target See all IL-27 (IL27) Antibodies
    IL-27 (IL27) (Interleukin 27 (IL27))
    Reactivity
    • 48
    • 44
    • 11
    • 1
    Mouse
    Host
    • 57
    • 13
    • 5
    • 2
    Rat
    Clonality
    • 61
    • 16
    Monoclonal
    Conjugate
    • 47
    • 6
    • 5
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IL-27 antibody is un-conjugated
    Application
    • 58
    • 38
    • 17
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse and rat p28 protein. Cross-reactivity with IL-27 from other sources has not been determined.
    Characteristics
    Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.
    Purification
    purified
    Sterility
    Sterile filtered
    Immunogen
    This Protein A purified monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.
    Immunogen Type: Recombinant Protein
    Clone
    3H12-F10
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product IL27 Primary Antibody
  • Application Notes
    IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.
    Comment

    Gene Name: IL27

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Expiry Date
    12 months
  • Murugaiyan, Mittal, Weiner: "Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11495-500, (2010) (PubMed).

  • Target
    IL-27 (IL27) (Interleukin 27 (IL27))
    Alternative Name
    IL-27/p28 (IL27 Products)
    Synonyms
    IL-27 antibody, IL-27A antibody, IL27A antibody, IL27p28 antibody, IL30 antibody, p28 antibody, RGD1561420 antibody, IL27 antibody, IL-27p28 antibody, Il30 antibody, interleukin 27 antibody, IL27 antibody, Il27 antibody
    Background
    Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.
    Synonyms: Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30
    Gene ID
    246779
    NCBI Accession
    NP_663611
    UniProt
    Q8K3I6
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