Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody (Atto 532)

Details for Product No. ABIN1043943
Antigen
Epitope
Heavy & Light Chain
840
233
200
38
13
8
Reactivity
Goat
222
198
165
143
123
76
75
57
53
53
44
41
40
35
23
14
13
7
7
7
3
2
Host
Rabbit
576
498
185
44
36
32
7
7
1
Clonality
Polyclonal
Conjugate
Atto 532
181
161
138
131
129
127
46
35
32
29
28
24
16
11
9
9
9
9
7
6
5
5
5
5
5
5
5
5
4
4
4
4
2
2
2
2
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
Options
Immunogen

Immunogen: Goat IgG whole molecule

Isotype IgG
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
Cross-Reactivity (Details) Pre-Adsorbed
Characteristics Anti-Goat IgG (H&L) conjugated to ATTO 532 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification Goat IgG (H&L) conjugated to ATTO 532 was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.  No reaction was observed against Human, Mouse or Rabbit Serum Proteins.  This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
Labeling Ratio 3.2
Antigen
Background

Synonyms: rabbit anti-Goat IgG ATTO 532 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO532 Conjugation

Background: Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Notes

Application Note: Anti-Goat IgG (H&L) conjugated to ATTO 532 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Comment

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) (ABIN1043943) Western Blot of Atto 532 conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. ...
 image for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) (ABIN1043943) Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) (Image 2)
Product cited in: Sarmento, Oneto, Pelicci, Pesce, Scipioni, Faretta, Furia, Dellino, Pelicci, Bianchini, Diaspro, Lanzanò: "Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures." in: Nature communications, Vol. 9, Issue 1, pp. 3415, 2018 (PubMed).