Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody (Atto 532)

Details for Product No. ABIN1043943, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
6012
1904
1869
1159
274
267
266
124
33
30
30
15
12
12
12
12
11
7
3
2
1
1
1
1
1
1
1
Reactivity
Goat
2740
2674
2192
1495
1369
700
664
447
434
362
359
354
275
258
257
147
115
84
43
26
24
21
12
12
8
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
Host
Rabbit
6469
4815
1755
887
458
274
87
54
43
35
22
8
6
5
4
1
1
1
Clonality
Polyclonal
Conjugate
Atto 532
2064
1741
1639
1144
678
644
422
315
171
122
102
102
94
89
88
88
86
84
79
78
74
73
70
68
68
67
65
65
63
55
55
54
54
54
54
54
53
53
53
53
53
48
47
47
41
40
32
31
30
23
23
22
20
19
18
17
16
15
15
15
14
14
13
12
12
12
12
10
10
10
10
10
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
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Immunogen

Immunogen: Goat IgG whole molecule

Isotype IgG
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
Cross-Reactivity (Details) Pre-Adsorbed
Characteristics Anti-Goat IgG (H&L) conjugated to ATTO 532 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification Goat IgG (H&L) conjugated to ATTO 532 was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.  No reaction was observed against Human, Mouse or Rabbit Serum Proteins.  This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
Labeling Ratio 3.2
Antigen
Background

Synonyms: rabbit anti-Goat IgG ATTO 532 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO532 Conjugation

Background: Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Application Notes

Application Note: Anti-Goat IgG (H&L) conjugated to ATTO 532 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Comment

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) (ABIN1043943) Western Blot of Atto 532 conjugated Rabbit anti-Goat IgG antibody. Lane 1: Goat IgG. ...
 image for Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) (ABIN1043943) Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) (Image 2)
Product cited in: Sarmento, Oneto, Pelicci, Pesce, Scipioni, Faretta, Furia, Dellino, Pelicci, Bianchini, Diaspro, Lanzanò: "Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures." in: Nature communications, Vol. 9, Issue 1, pp. 3415, 2018 (PubMed).