Rabbit anti-Mouse (Murine) IgG Antibody (Atto 550)

Details for Product No. ABIN1043989, Supplier: Log in to see
Antigen
Reactivity
Mouse (Murine)
2740
2673
2192
1495
1370
700
664
447
434
362
359
354
275
258
257
147
115
84
43
26
24
21
12
12
8
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
Host
Rabbit
6469
4815
1755
887
458
274
87
54
43
35
22
8
6
5
4
1
1
1
Clonality
Polyclonal
Conjugate
Atto 550
2064
1741
1639
1144
678
644
422
315
171
122
102
102
94
89
88
88
86
84
79
78
74
73
70
68
68
67
65
65
63
55
55
54
54
54
54
54
53
53
53
53
53
48
47
47
41
40
32
31
30
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
10
10
10
10
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
FLISA, Fluorescence Microscopy (FM), Western Blotting (WB)
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Immunogen

Immunogen: highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins

Immunogen Type: Native Protein

Isotype IgG
Characteristics Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 550 was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Labeling Ratio 1.5
Antigen
Background

Synonyms: rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO550 conjugation, rabbit anti-Mouse IgG ATTO550 conjugated Antibody

Background: Anti-Mouse IgG ATTO 550 Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG1, IgG2a, IgG2b and IgG3 chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Application Notes

Application Note: Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Comment

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.
Storage RT,4 °C,-20 °C
Storage Comment Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
Expiry Date 12 months
Supplier Images
 image for Rabbit anti-Mouse (Murine) IgG antibody (Atto 550) (ABIN1043989) Rabbit anti-Mouse (Murine) IgG antibody (Atto 550)
Western Blotting (WB) image for Rabbit anti-Mouse (Murine) IgG antibody (Atto 550) (ABIN1043989) Western Blot of ATTO 550 Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, 3) secondary antibod...
 image for Rabbit anti-Mouse (Murine) IgG antibody (Atto 550) (ABIN1043989) Rabbit anti-Mouse (Murine) IgG antibody (Atto 550) (Image 3)