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Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
Catalog No. ABIN1043994
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 691
    • 672
    • 559
    • 452
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 95
    • 95
    • 92
    • 62
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 1718
    • 1473
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Rabbit
    Clonality
    • 3346
    • 164
    • 1
    Polyclonal
    Conjugate
    • 556
    • 408
    • 406
    • 366
    • 298
    • 276
    • 228
    • 120
    • 99
    • 67
    • 66
    • 62
    • 62
    • 52
    • 48
    • 47
    • 44
    • 43
    • 43
    • 41
    • 39
    • 32
    • 32
    • 32
    • 31
    • 31
    • 30
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    Biotin
    Application
    • 1836
    • 1788
    • 1787
    • 1498
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
    Characteristics
    Mouse secondary antibody conjugated to biotin is available in a variety of formats. Anti-IgG Secondary Antibody conjugate is Suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Mouse IgG whole molecule

    Isotype
    IgG
    Labeling Ratio
    10-20
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Mouse secondary antibody conjugated to biotin is available in a variety of formats. Anti-IgG Secondary Antibody conjugate is Suitable for western blot, ELISA and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:200,000 - 1:300,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Comment

    When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid cycles of freezing and thawing.
    This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: rabbit anti-Mouse IgG Antibody biotin conjugation, rabbit anti-Mouse IgG biotin conjugated Antibody

    Background: Anti-Mouse IgG Biotin Antibody generated in rabbit detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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