Rabbit anti-Cow IgG (Heavy & Light Chain) Antibody (Texas Red (TR)) - Preadsorbed

Catalog No. ABIN1044003

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: Texas Red (TR)

Application: Fluorescence Microscopy (FM)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Bovine IgG and Bovine Serum.

Characteristics: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Bovine IgG whole molecule

Isotype: IgG

Labeling Ratio: 1.6

Application Details

Application Notes:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

IF Microscopy Dilution: 1:500-1:2,500

Comment:

Texas Red™ is a registered trademark of Molecular Probes Inc.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Product is photosensitive and should be protected from light.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to opening. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -24 °C or below.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Rabbit F(ab')2 Anti-Bovine Texas Red™ Conjugated Antibody, Rabbit Fab2 Anti-Bovine Texas Red™ Conjugated Antibody, Rabbit Fab'2 Anti-Bovine Antibody Texas Red™ Conjugation

Background: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.