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Rabbit anti-Horse IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated F(ab')2 fragment
Catalog No. ABIN1044026
  • Target See all IgG products
    IgG
    Fragment
    F(ab')2 fragment
    Binding Specificity
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 692
    • 672
    • 559
    • 452
    • 366
    • 239
    • 198
    • 147
    • 137
    • 130
    • 122
    • 98
    • 95
    • 95
    • 92
    • 62
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Horse
    Host
    • 1718
    • 1473
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Rabbit
    Clonality
    • 3346
    • 164
    • 1
    Polyclonal
    Application
    • 1836
    • 1788
    • 1787
    • 1498
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Horse IgG and Horse Serum.
    Purification
    Preadsorption: immunoaffinity chromatography using Horse IgG coupled to agarose beads
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Horse IgG whole molecule

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000-1:5,000

    Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

    ELISA Dilution: 1:20,000-1:100,000

    Western Blot Dilution: 1:2,000-1:10,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Centrifuge product if not completely clear after standing at room temperature.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: Rabbit F(ab')2 Anti-Horse IgG Antibody, Rabbit F(ab')2 Anti-Horse IgG Antibody, Rabbit Fab2 Anti-Horse IgG Antibody

    Background: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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