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Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed Secondary Antibody

FLISA, FACS, FM Polyclonal IgG
Catalog No. ABIN1044086
$276.00
Plus shipping costs $45.00
500 μL
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  • Target
    Binding Specificity
    Heavy & Light Chain
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    TRITC
    Application
    FLISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.
    Characteristics
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Human IgG whole molecule

    Isotype
    IgG
    Fragment
    Fab fragment
    Labeling Ratio
    1.3
  • Application Notes

    Application Note: Fab Anti-Human IgG (H&L) Rhodamine Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Handling Advice
    Avoid cycles of freezing and thawing.
    Product is photosensitive and should be protected from light.
    Centrifuge product if not completely clear after standing at room temperature.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Expiry Date
    12 months
  • Target
    Background

    Synonyms: Rabbit Fab Anti-Human IgG Antibody Rhodamine Conjugation, Rabbit Fab Anti-Human IgG TRITC Conjugated Antibody

    Background: Fab Anti-Human IgG (H&L) Rhodamine Antibody generated in rabbit detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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