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NFYB antibody (N-Term)

NFYB Reactivity: Human ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN104771
  • Target See all NFYB Antibodies
    NFYB (Nuclear Transcription Factor Y, beta (NFYB))
    Binding Specificity
    • 15
    • 9
    • 5
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 43
    • 20
    • 17
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 38
    • 5
    Rabbit
    Clonality
    • 38
    • 5
    Polyclonal
    Conjugate
    • 26
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NFYB antibody is un-conjugated
    Application
    • 34
    • 22
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    NF-Y (B subunit) peptide corresponding to a region near the of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
    Isotype
    IgG
    Top Product
    Discover our top product NFYB Primary Antibody
  • Application Notes
    This product was assayed by immunoblot and found to be reactive against the 25 kDa B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] Goat) Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (B subunit specific).  Minimal reaction was observed by immunoblot against the 35 kDa A subunit of NF-Y.  This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 µl per assay.  This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS substrate.  A dilution of 1:4000 is suggested from this experiment.  Minimal reactivity was observed by ELISA against the A subunit of NF-Y. 
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Manni, Caretti, Artuso, Gurtner, Emiliozzi, Sacchi, Mantovani, Piaggio: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, (2008) (PubMed).

  • Target
    NFYB (Nuclear Transcription Factor Y, beta (NFYB))
    Abstract
    NFYB Products
    Synonyms
    CBF-A antibody, CBF-B antibody, HAP3 antibody, NF-YB antibody, NFYB antibody, nfyb antibody, zgc:110533 antibody, zgc:110552 antibody, hap3 antibody, cbf-a antibody, cbf-b antibody, CBFB antibody, Nfya antibody, AA985999 antibody, Cbf-A antibody, nuclear transcription factor Y subunit beta antibody, nuclear transcription factor Y subunit beta L homeolog antibody, nuclear transcription factor Y, beta b antibody, nuclear transcription factor Y, beta a antibody, nuclear transcription factor-Y beta antibody, NFYB antibody, nfyb.L antibody, nfybb antibody, nfyba antibody, nfyb antibody, Nfyb antibody
    Background
    Synonyms: Nuclear transcription factor Y subunit B
    Gene ID
    4801
    UniProt
    P25208
    Pathways
    Regulation of Lipid Metabolism by PPARalpha
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