Dopamine d2 Receptor antibody (Cytoplasmic Domain)

Catalog No. ABIN1048503

Product Details anti-Dopamine d2 Receptor Antibody

Target: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Binding Specificity: Cytoplasmic Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dopamine d2 Receptor antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DRD2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ZNF57 (42 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset (100%) Gibbon, Monkey, Rabbit (95%) Dog, Bovine, Panda, Horse, Pig (89%) Mouse, Rat, Ferret, Bat, Hamster, Elephant (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human DRD2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset (100%), Gibbon, Monkey, Rabbit (95%), Dog, Bovine, Panda, Horse, Pig (89%), Mouse, Rat, Ferret, Bat, Hamster, Elephant (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Dopamine d2 Receptor

Target: Dopamine d2 Receptor (DRD2) (Dopamine Receptor D2 (DRD2))

Alternative Name: DRD2 / Dopamine Receptor D2 (DRD2 Products)

Synonyms: D2DR antibody, D2R antibody, drd2b antibody, d2r2 antibody, Drd-2 antibody, d2dr antibody, d2r antibody, drd2 antibody, drd2-B antibody, DRD2 antibody, D2 antibody, CG18741 antibody, CG7569 antibody, DAMB antibody, DDR2 antibody, DOPR2 antibody, DOPR99B antibody, Dar2 antibody, Dmel\\CG18741 antibody, DopD1 antibody, DopR99B antibody, DopRII antibody, dopamine receptor D2 antibody, dopamine receptor D2 like antibody, dopamine receptor D2 L homeolog antibody, dopamine receptor D2 S homeolog antibody, Dopamine 1-like receptor 2 antibody, DRD2 antibody, drd2l antibody, drd2 antibody, Drd2 antibody, drd2.L antibody, drd2.S antibody, Dop1R2 antibody

Background: Name/Gene ID: DRD2
Subfamily: Dopamine
Family: GPCR

Synonyms: DRD2, D2 dopamine receptor, D2R, Dopamine receptor D2 isoform, Dopamine 2 receptor, D(2) dopamine receptor, D2DR, Dopamine D2 receptor, Dopamine receptor D2

Gene ID: 1813

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity