Metabotropic Glutamate Receptor 5 antibody (Glutamate Receptor, Metabotropic 5) (C-Term)

Details for Product anti-GRM5 Antibody No. ABIN1048937
Target
Binding Specificity
C-Term
82
52
15
12
10
9
7
3
3
2
2
1
1
1
1
1
Reactivity
Bat, Cow, Dog, Horse, Human, Monkey, Mouse, Rat
179
147
138
6
6
6
5
5
3
2
2
2
2
2
1
Host
Rabbit
91
88
4
2
Clonality
Polyclonal
Conjugate
This Metabotropic Glutamate Receptor 5 antibody is un-conjugated
12
10
7
6
6
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
151
107
82
66
57
37
31
13
12
4
4
3
2
1
Options
Brand IHC-plus™
Immunogen Synthetic 20 amino acid peptide from C-terminus of human GRM5 / MGLUR5. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Horse (100%), Platypus (95%), Opossum, Turkey, Chicken, Lizard (90%).

Type of Immunogen: Synthetic peptide
Specificity Human GRM5 / MGLUR5. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except PLEC (45 % ).
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Bat, Bovine, Horse (100%) Platypus (95%) Opossum, Turkey, Chicken, Lizard (90%).
Purification Immunoaffinity purified
Target
Alternative Name GRM5 / MGLUR5 (GRM5 Antibody Abstract)
Background Name/Gene ID: GRM5
Subfamily: Metabotropic glutamate
Family: GPCR

Synonyms: GRM5, GPRC1E, MGLUR5, Mglur5b, MGlu5
Gene ID 2915
Pathways Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity
Application Notes Approved: IHC, IHC-P (4 - 13 μg/mL), WB
Comment

Target Species of Antibody: Human

Assay Procedure

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months
Images
Image no. 1 for anti-Glutamate Receptor, Metabotropic 5 (GRM5) (C-Term) antibody (ABIN1048937) Anti-GRM5 / MGLUR5 antibody ABIN1048937 IHC staining of human brain, neurons and gli...