MAP3K8 antibody (N-Term)

Catalog No. ABIN1049035

Product Details anti-MAP3K8 Antibody

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Binding Specificity: N-Term

Reactivity: Human, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K8 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human MAP3K8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine (100%) Hamster, Panda, Bat, Dog (95%) Mouse, Rat, Rabbit, Pig (89%) Elephant, Horse, Platypus (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from near N-terminus of human MAP3K8. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine (100%), Hamster, Panda, Bat, Dog (95%), Mouse, Rat, Rabbit, Pig (89%), Elephant, Horse, Platypus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (15 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for MAP3K8

Target: MAP3K8 (Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8))

Alternative Name: MAP3K8 / TPL2 (MAP3K8 Products)

Synonyms: MAP3K8 antibody, COT antibody, EST antibody, ESTF antibody, MEKK8 antibody, TPL2 antibody, Tpl-2 antibody, c-COT antibody, Cot antibody, Cot/Tpl2 antibody, Est antibody, Estf antibody, Tpl2 antibody, D17TPL2 antibody, mitogen-activated protein kinase kinase kinase 8 antibody, MAP3K8 antibody, map3k8 antibody, Map3k8 antibody

Background: Name/Gene ID: MAP3K8
Subfamily: MAP3K
Family: Protein Kinase

Synonyms: MAP3K8, C-COT, Cancer Osaka thyroid oncogene, COT, Ewing sarcoma transformant, EST, MEKK8, Proto-oncogene c-Cot, TPL2, Tumor progression locus 2, Protein kinase cot, Tpl-2, ESTF

Gene ID: 1326

Pathways: PI3K-Akt Signaling, TCR Signaling