P2RY8 antibody (C-Term)

Catalog No. ABIN1049223

Product Details anti-P2RY8 Antibody

Target: P2RY8 (Purinergic Receptor P2Y, G-Protein Coupled, 8 (P2RY8))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RY8 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human P2RY8 / P2Y8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%) Gorilla (95%).

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic 20 amino acid peptide from C-terminus of human P2RY8 / P2Y8.

Application Details

Comment:

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: PBS, 0.1% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Long term: -70°C, Short term: +4°C

Target Details for P2RY8

Target: P2RY8 (Purinergic Receptor P2Y, G-Protein Coupled, 8 (P2RY8))

Alternative Name: P2RY8 / P2Y8 (P2RY8 Products)

Synonyms: P2Y8 antibody, P2Y purinoceptor 8 antibody, P2Y receptor family member 8 antibody, purinergic receptor P2Y8 antibody, p2ry8 antibody, P2RY8 antibody

Background: Standard Gene Symbol: P2RY8, Gene Family: GPCR, Gene Subfamily: Orphan-A, Synonyms: P2RY8, p2RY8, p2Y purinoceptor 8, p2Y8

Gene ID: 286530

UniProt: Q86VZ1