TLK1 antibody (Isoform 1)

Catalog No. ABIN107632

Product Details anti-TLK1 Antibody

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Binding Specificity: AA 737-751, Isoform 1

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 737-751 of human Tlk1 (Tousled-like kinase) protein.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 86 kDa, 89 kDa or 64 kDa in size corresponding to isoforms of TLk-1 by western blotting in the appropriate cell lysate or extract.  This antibody reacts with both phosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) of the protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.60 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for TLK1

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Alternative Name: Tlk1 (TLK1 Products)

Synonyms: PKU-beta antibody, 4930545J15Rik antibody, fe11e12 antibody, si:dkey-251e16.4 antibody, tlk1 antibody, wu:fe11e12 antibody, TLK1 antibody, fb76h12 antibody, si:dkey-88m1.1 antibody, wu:fb76h12 antibody, DKFZp459L163 antibody, tousled like kinase 1 antibody, tousled-like kinase 1 antibody, tousled-like kinase 1b antibody, tousled like kinase 1 like antibody, serine/threonine-protein kinase TOUSLED antibody, tousled-like kinase 1a antibody, Serine/threonine-protein kinase tousled-like 1 antibody, TLK1 antibody, Tlk1 antibody, tlk1b antibody, TLK1L antibody, LOC542181 antibody, tlk1a antibody, tlk1 antibody, tlk-1 antibody

Background: Tlk-1 (also known as tousled-like kinase, serine/threonine-protein kinase tousled-like 1 and PKU-beta) is a target for Chk1 upon induction of DNA-damage. Tousled-like kinases, first described in Arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly. Tlk-1 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent.  Tlk-1 heterodimerizes with Tlk-2.  Multiple isoforms of the protein have been detected.  Mature Tlk-1 is reported to have an apparent molecular weight of ~86kDa, ~89kDa and ~64kDa corresponding to isoforms 1, 2 and 3 respectively.
Synonyms: PKU beta antibody, serine threonine protein kinase antibody, Serine/threonine protein kinase tousled like 1 antibody, TLK1 antibody, Tousled like kinase 1 antibody

Gene ID: 9874, 33636698

UniProt: Q9UKI8