PRICKLE1 antibody (C-Term)

Catalog No. ABIN1077370

Product Details anti-PRICKLE1 Antibody

Target: PRICKLE1 (Prickle-Like Protein 1 (PRICKLE1))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRICKLE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Specificity: Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.

Purification: Affinity chromatography purified via peptide column

Immunogen: PRICKLE1 antibody was raised against a AA 19 synthetic peptide near the carboxy terminus of human PRICKLE1.

Isotype: IgG

Application Details

Application Notes: PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. Optimal dilutions for each application to be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: 4 °C/-20 °C

Storage Comment: PRICKLE1 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year.

Expiry Date: 12 months

Target Details for PRICKLE1

Target: PRICKLE1 (Prickle-Like Protein 1 (PRICKLE1))

Alternative Name: PRICKLE1 (PRICKLE1 Products)

Background: Synonyms:
REST, NRSF-interacting LIM domain protein 1, RILP, EPM1B

PRICKLE1 is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4. It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.

Gene ID: 144165

NCBI Accession: NP_694571

Pathways: WNT Signaling, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development, Protein targeting to Nucleus