alpha Tubulin antibody (N-Term)

Catalog No. ABIN1077421

Product Details anti-alpha Tubulin Antibody

Target: alpha Tubulin (TUBA1)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Affinity chromatography purified via peptide column

Immunogen: Rabbit polyclonal Tubulin antibody was raised against a 16 amino acid peptide near the amino terminus of human Tubulin.

Isotype: IgG

Application Details

Application Notes: Tubulin antibody can be used for detection of Tubulin by Western blot at 0.5 and 1 μg/mL.

Comment:

(NT) Tubulin alpha-1A

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: 4 °C

Storage Comment: Alpha-tubulin antibody can be stored at 4 °C for three months. At -20 °C stable for up to one year.

Expiry Date: 12 months

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: alpha Tubulin (TUBA1 Products)

Synonyms: Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody

Background: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.
Synonyms: Tubulin alpha-1A, TUBA1A, TUBA3, LIS3

Molecular Weight: 50 kDa (calculated)

Gene ID: 7278

NCBI Accession: NP_005992

Pathways: Microtubule Dynamics