COL3A1 antibody (AA 1171-1435)

Catalog No. ABIN1077676

Product Details anti-COL3A1 Antibody

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Binding Specificity: AA 1171-1435

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL3A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Collagen Type III Alpha 1 (COL3a1)

Specificity: The antibody is a rabbit polyclonal antibody raised against COL3a1. It has been selected for its ability to recognize COL3a1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Collagen Type III Alpha 1 (COL3a1) corresdonding to Arg1171~Arg1435 (Accession # P13941)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for COL3A1

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Alternative Name: Collagen Type III Alpha 1 (COL3A1 Products)

Synonyms: EDS4A antibody, AW550625 antibody, Col3a-1 antibody, MMS10-W antibody, Ms10w antibody, mKIAA4231 antibody, col3a1 antibody, collagen type III alpha 1 chain antibody, collagen, type III, alpha 1 antibody, collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) S homeolog antibody, COL3A1 antibody, Col3a1 antibody, col3a1 antibody, col3a1.S antibody

Background: COL3-A1, COL3A1, EDS4A, Collagen Type III Alpha 1, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, Collagen Alpha-1(III)chain

Molecular Weight: 17.1 kDa

Pathways: Autophagy, Growth Factor Binding