BID antibody (AA 1-195)

Catalog No. ABIN1077862

Product Details anti-BID Antibody

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Binding Specificity: AA 1-195

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to BH3 Interacting Domain Death Agonist (Bid)

Specificity: The antibody is a rabbit polyclonal antibody raised against Bid. It has been selected for its ability to recognize Bid in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant BH3 Interacting Domain Death Agonist (Bid) corresdonding to Met1~Asp195 (Accession # P70444)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:100-400 Immunohistochemistry: 5-20 μg/mL

1:10-40 Immunocytochemistry: 5-20 μg/mL

1:10-40 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BH3 Interacting Domain Death Agonist (BID Products)

Synonyms: FP497 antibody, 2700049M22Rik antibody, AI875481 antibody, AU022477 antibody, BID antibody, bid antibody, si:ch211-238n5.6 antibody, fp497 antibody, xbid antibody, DKFZp469E066 antibody, BH3 interacting domain death agonist antibody, BH3 interacting domain death agonist S homeolog antibody, BID antibody, Bid antibody, bida antibody, bid.S antibody, bid antibody

Background: P22 BID

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity